| IntroductionThe neurological dysfunction leaved over by ischemic ce rebrovascular disease has been a difficult problems of huma n medicine.With the Profound investigation into cerebral a rterial thrombosis,it could induce the injury of ischemia-reperfusion after bloodstream reviving re-canalization.The therapeutic angiogenesis established a new channel to angi ogenesis of ischemia cerebral vascular disease.Therefore,it is necessary to properly control the proc ess of therapeutic angiogenesis which is effective to impro ve cerebral ischemia.The process of angiogenesis mainly in clude two regulated channels:one is the vascular endotheli al growth factor(VEGF) and its receptor(flt-1,etc.) regu late access,and the other is angiopoietin-1(angiopoietin, Ang) and its receptor(Tie) regulating access,the two chan nels work together to promote angiogenesis.Vascular endoth elial growth factor is a kind of cytokines to promote angio genesis best as we know.The activation of VEGF-VEGFR syste m is to cause hyperplasia endothelial cells and to induce n eovascularization.The new vessels,extending from normal b rain tissue to ischemic region,augment reperfusion and ven tilation of brain tissue to be involved in and lessen ische mia-reperfusion injury.The Ang-1/Tie-2 regulation pathway has significant meaning for the maturity and stability of b lood vessel in the process of angiogenesis.Angiopoietin-1 (Ang-1) could inhibit vascular endothelial cell apoptosis, reducing the Leakage of blood-brain barrier after cerebral ischemia in order to reduce the infarct brain volume,and p romote the budding of vascular endothelial cells.Used the MCAO model,this research approached the mechanism of early acupuncture treating resisting cerebral ischemia-reperfusio n injury and based on the observation of the changes of the VEGFmRNA,Ang-1mRNA/Tie-2 and angiogenesis.Meterials and MethodsSD rats were randomly divided into the normal control g roup,sham operation group,model group(ischemia-reperfusio n 1day,2days,4days,8days),electro-acupuncture treatment group(ischemia-reperfusion 1day,2days,4days,8days and ad d electro-acupuncture treatment).We used the suture in MCA to establish MCAO isehemia-reperfusion model.The remarks of nerve function deficit referring to Lundy remarking stan dards,Only the rats with over one nerve function deficits can be retained.The acupoints of "BAIHUI","SHUIGOU" and "ZUSANLI" were selected,stimulated with nondense and dens e wave for 30 minutes,observed isehemia brain tissue,dete cted the change of the expression of Tie-2 and microvessel density(MVD) in right cerebral cortex by immunohistochemist ry method,observed the expression of Tie-2 ischemia brain tissue,detected the change of the expression of VEGFmRNA a nd Ang-1mRNA in right cerebral cortex by in situ hybridizat ion method,and analyzed the intervention of time change an d the effects of electro-acupuncture among each indicators. All the datum were processed by SPSS11.0 statistics softwa re package and all results were demonstrated by the average and standard deviation.Results and DiscussionRats in electro-acupuncture group and model group have varying degrees of nerve function deficit.Rats in each sub -groups of electro-acupuncture group have lower nerve funct ion deficit scores than the corresponding sub-group of mode 1 group.This shows the electro-acupuncture could significa ntly improve the nerve missing sign of the rats with cerebr a1 ischemia reperfusion.Rats in each sub-groups of both electro-acupuncture gro up and model group can appear microvascular proliferation i n ipsilateral cerebral cortex tissue,and rats in each phas e of the electro-acupuncture group have higher microvessel density than the model group.This shows electro-acupunctur e could promote angiogenesis after cerebral ischemia-reperf usion.The expression of VEGFmRNA in ischemic ipsilateral cere bral cortex of both model group rats and electro-acupunctur e group rats were significantly higher than those of normal and sham operation group rats;while the expression of VEG FmRNA in ischemic ipsilateral cerebral cortex of electro-ac upuncture group rats were significantly higher than those o f corresponding phase rats of model group.This shows elect ro-acupuncture can increase the expression level of VEGFmRN A after cerebral ischemia-reperfusion in ipsilateral cerebr al cortex.The expression of Ang-1mRNA and Tie-2 in ischemic ipsil ateral cerebral cortex after 2,4 or 8 days reperfusion of both model group rats and electro-acupuncture group rats we re significantly higher than those of normal and sham opera tion group rats;while the expression ofAng-1mRNA and Tie-2 in ischemic ipsilateral cerebral cortex of electro-acupunc ture group rats were significantly higher than those of cor responding phase rats of model group.This shows electro-ac upuncture can increase the expression level of Ang-lmRNA an d Tie-2 after cerebral ischemia-reperfusion in ipsilateral cerebral cortex.Angiogenesis can arise after cerebral ischemia-reperfus ion.VEGF and Ang-1/Tie-2 participate in angiogenesis after cerebral ischemia-reperfusion injury.Electro-acupuncture adjust the process of angiogenesis in brain cortex after ce rebral ischemia-reperfusion injury,can effect urge angioge nesis in ischemia brain tissue and reduce the degree of ner ve function deficit.This could be carried by activating th e expression of VEGFmRNA and Ang-1mRNA/Tie-2. |
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