Effect Of Exercise And Drugs On Biomechanical Properties Of The Bone On SD Female Rats

Objective:with aging, people, especially women aroused much attention by the reduction of osseous performance which because of the estrogenic decrease. This thesis tries to use the older female rats SD to simulate on the condition of non-ovary and to make research using the interference of moments and medicines, which aim at discussing moments'and drugs'function to prevent and treat osteoporosis which result from hormone decrease because of aging as well as make the academic reference for women's choices to moderate athletics and medicines.Methods:to group randomly 60 female rats SD who 3 months old into the operation group and fake group, I divide the operation group into the operation stillness team of 10 rats (OVX), the operation giving-medicine stillness team of 10 rats (OVX+ES), the operation giving-medicine moment team of 10 rats (OVX+ES+EX), the operation pure action team of 10 rats (OVX+ES), the fake operation action team of 10 rats (SHAM+EX), the fake operation stillness team of 10 rats(SHAM). The operation group is that ovary-removed on both sides, the fake operation group is that remove ovary-size fats near ovary. one weeks latter, the moment groups all utilize the machine of little animal running platform (model: DSPT—202) to run an hour daily that 18m/min. The running platform take on the level circumstance and the frequency is 6 days one week. The medicines to the giving-medicine group is 17—βestradiol which dosage is 5ug/200g weight. The time to inject is Monday, Wednesday and Friday. The moment group carries through the preliminary training that is increasing intensity to study run at the running platform until measure up to requires. To hocus rats with Hydration of acetaldehyde and measure its weight, kill all rats by bleed at the femoral and take their right and left shankbones of back crura and thighbone,the fourth lumbar vertebra, then remove the clear connective tissue. After that, lay the shankbone into the experiment oven which temperature is 60 degree Celsius by number and take them out 12 hours latter. Then measure all shankones'masses by electronic analytical balance and measure their lengths by vernier caliper, then calculate the average of the right and left shankbones and the ratio of the rats'masses which multiply 100----the index of them. Then bandage the thighbones and lumbar spine bones with the gauze that is penetrated solution pbs, and reserve within the icebox which is -20 degree Celsius, as melt toward the room temperature under the normal temperature, then after measure the masses of right thighbone and the fourth lumbar vertebra by DEXA, measure the geometry data of the fourth lumbar vertebra, using the universal testing machine to carry out the three-points-bend experiment over the femoral and the condensing test of the lumbar bone. And paste in the rupturing thighbone with adhesive, after saw evenly the rupturing place by dental saw, to measure the femoral inner and outer lengths with vernier caliper and calculate the sectional moment of inertia, lastly calculate the indicators of bone material mechanics. Mathematical Statistics adopts SPSS12.0 to carry out the analysis of variance in single factor.Results:according to this experiment, the ovary-removed rats'skeletal geometry, bone mineral density and mechanical index value took place the tendency to descend(P<0.01) in comparison with the fake operation group. The indicators of bone mineral density and the bone biomechanical indicators of ovary-removed rats who experienced the interference of medicines and movements had not change dramatically in comparison with the fake operation group, which meant medicines and movements work to prevent osteoporosis leading by the lack of estrogen, but the effect was not alone. Because the ovary-removed rats of the operation-medicine group and operation-movement group had changed dramatically compared with ones of the operation-movement-medicine group( (P<0.01 or P<0.05), despite its mechanical index and bone mineral density values had a little diversification compared with the emasculation group and stillness group( (P<0.01 or P<0.05). The fake operation group is to research whether the normal surgery lead to the change of skeletal dynamo data of rats. the ovary-removed operation lead to the change of skeletal dynamo data of rats. The above-mentioned results revealed that the estrogen on emasculation rat bone morphology, bone mineral density and bone biomechanical has a certain therapeutic effect. By the same reason, running matters to enhance the bone masses, and running blending with 17—βestradiol have the coordinating effect to treat the rats who suffer from osteoporosis. Key word: exercise and drug, drug, exercise, rag, bone mineral density, femur, lumbar spine bone, tibia, mechanical indicator.Conclusion:17-βestrodiol and running can improve length of the tibia and index of the mass.But 17-βestrodiol plus running can not develop them each other.17-βostradiol plus running in effect improve BMD of the thighbone and vertebra of the emasculated rats.Only 17-βestrodiol only running also improve BMD .But less notable than drugs plus running.Only17-βestrodiol only running improve biomechanical index of the thighbone and lumbar bone partly.But 17-βostrodiol plus running can in effect improve the biomechanical index of them.Experimental results show esteoporosis was prevented and cured when agedness came in,intervenor of only drugs and running was less effective than intervernor drugs plus running.