| Objective:To Explore the effect of tanshinone and western medicine of phosphocreatine sodium to myocardium of exhaustive exercise rats by observing exhaustive exercise rat SOD and MDA of blood-serum and myocardium tissues and the change of Bax and Bcl-2 of Apoptosis correlation factor and analyzing the ultramicrostructure change of myocardium.Methods:250g±10g body weight of male SD rats were randomly divided into six groups:normal control group,exhaustive exercise model group,exhaustive exercise+low-dose tanshinone group,exhaustive exercise+middle-dose tanshinone group,exhaustive exercise +hypso-dose tanshinone group and exhaustive exercise+western medicine of phosphocreatine sodium group of 8.Normal control group:untake any exercise,common breed;exhaustive exercise model group:take swimming exercise for endurance;the rest groups:intraperitoneal inject drug after swimming exercise for endurance. Different dose tanshinone groups were given tanshinone of intraperitoneal injection low-dose3mg/kg/d,middle-dose6mg/kg/d,hypso-dosel2mg/kg/d after swimming exercise for endurance;exhaustive exercise+western medicine of phosphocreatine sodium group were given phosphocreatine sodium of intraperitoneal injection 72mg/kg/d after swimming exercise for endurance.After six weeks experiment of swimming exercise for endurance,all groups take blood-serum and myocardium tissues to freeze and preserve.Adopt immunohistochemical method to detect the proteinum expression of Bax and Bcl-2 of myocardium tissues and applicat spectrophotometric method detecting the content of SOD and MDA of blood-serum and myocardium tissues and observe the change of morphous structure of myocardium tissues by HE dyeing light microscope and transmission electron microscope and preserve the shot for analyzing.Results:1.The detection of SOD of blood-serum and myocardium tissues and the comparing result.1.1 The SOD of blood-serum and myocardium tissues of normal control group are discerned 128.49±9.72 and 66.86±4.97;comparing to normal control group,the activity of SOD of blood-serum and myocardium tissues of exhaustive exercise model group are discerned 77.17±12.77 and 26.30±4.72 (P<0.01),cutting down obviously.1.2 Comparing to exhaustive exercise model group,the activity of SOD of blood-serum and myocardium tissues of low-dose, middle-dose and hypso-dose tanshinone all heightenig obviously. The activity of SOD of blood-serum are discerned 105.28±6.66,118.36±8.88 and 108.30±7.67(P< 0.01);The activity of SOD of myocardium tissues are discerned 51.73±5.42,62.55±5.27 and 56.78±3.69(P<0.01).1.3 Comparing to exhaustive exercise model group,the activity of SOD of blood-serum and myocardium tissues of phosphocreatine sodium group are discerned 121.19±6.98 and 62.77±5.79(P<0.01),all heightening obviously.2. The detection of MDA of blood-serum and myocardium tissues and the comparing result.2.1 The MDA of blood-serum and myocardium tissues of normal control group are discerned 5.78±0.71 and 2.75±0.95;comparing to normal control group,the content of MDA of blood-serum and myocardium tissues of exhaustive exercise model group are discerned 11.80±2.35 and 11.17±1.24(P<0.01),heightenig obviously.2.2 Comparing to exhaustive exercise model group,the content of MDA of blood-serum and myocardium tissues of low-dose,middle-dose and hypso-dose tanshinone all cutting down obviously. The content of MDA of blood-serum are discerned 8.14±0.95,6.59±0.62 and 7.80±0.77(P<0.01);The content of MDA of myocardium tissues are discerned 5.29±1.06,3.91±1.00 and 4.98±1.02(P<0.01).2.3 Comparing to exhaustive exercise model group,the content of MDA of blood-serum and myocardium tissues of phosphocreatine sodium group are discerned 121.19±6.98 and 62.77±5.79(P<0.01),all cutting down obviously.3.Compare the results of SOD and MDA of all drug groups.Comparing the SOD and MDA of blood-serum and myocardium tissues among the groups of different doses of Chinese crude drug,there is no difference between low-dose and middle-dose(P>0.05),but middle-dose have difference and its effect to SOD and MDA surpass to other two groups,comparing to low-dose and middle-dose(P<0.05).Comparing the SOD and MDA of blood-serum and myocardium tissues between western medicine of phosphocreatine sodium group and middle-dose tanshinone group,there is no difference(P> 0.05);Comparing western medicine of phosphocreatine sodium group to low-dose and middle-dose tanshinone groups,it has difference and its effect to SOD and MDA surpass to other two groups.4. Observe morphology4.1 The result by observing light microscope.Normal control group:Heart muscle fiber arrange normally and cell nuclear is ellipse,locating center.Exhaustive exercise model group:Heart muscle fiber swell and arrange disorderly and distantly and its diastema widen;part of cell nucleus degenerate and cell interstitial substance bleed.Low-dose and hypso-dose tanshinone groups:Heart muscle fiber arrange distantly and cell interstitial substance bleed.Middle-dose tanshinone and western medicine of phosphocreatine sodium groups: Heart muscle fiber polarity arrange disorderly slightly.4.2 The result by observing electron microscope.Normal control group:Heart muscle fiber arrange normally,vacuolar degeneration lightly and myocardial cell nuclear and shape structure of chondriosome are normal.Exhaustive exercise model group:Heart muscle fiber atrophy,part dissolve and arrange disorderly;chondriosome hyperplasy,distribut disorderly;tunica muscularis ecphyma sawly and dropsy;Vascular proliferation.Low-dose tanshinone group:Heart muscle fiber arrange disorderly slightly and atrophy unobviously;chondriosome distribute uniformity.Middle-dose tanshinone group:The structure and arrangement of heart muscle fiber are normal basically.Hypso-dose tanshinone group:Part of heart muscle fiber fibronolysis and surrounding heart muscle fiber revive fundamentally.Western medicine of phosphocreatine sodium group:The chondriosome of heart muscle fiber mult and heart muscle fiber under tunica muscularis dissolve lightly.5. The result by detecting immunohistochemical method.Different doses of tanshinone and phosphocreatine sodium all restraint the expression of Bax and promote the expression of Bcl-2,but compering the proteinum expression of Bax and Bcl-2 among all grug groups,there is no significant deviation.Conclusion:l.The experiment adopt Thomas method copying rats' exhaustive swimming exercise and myocardial damagement pattern;The shape of myocardium tissues of exhaustive swimming exercise model group destroy obviously.Animal model taken by this method is stable,manipulateion strongly and reproducibility easily.2. Different doses of tanshinone and phosphocreatine sodium could in some degree elevate the activity of SOD and cut down the content of MDA of blood-serum and myocardium tissues of exhaustive swimming exercise rats,preserve myocardial damage of exhaustive swimming exercise rats in some degree accordingly.3. Different doses of tanshinone and phosphocreatine sodium could regulate the change of Bax and Bcl-2 of Apoptosis correlation factor and improve the condition of myocardial damage accordingly.4. Observing by morphology,different doses of tanshinone and phosphocreatine sodium lighten destruction of myocardium structure and relieve myocardial damage.5. Observing by morphology and connetting oxygen free radical and the change of Apoptosis index,Middle-dose tanshinone take protection of myocardial preservation further evident than other doses groups.6. Sum up above statement,tanshinone and phosphocreatine sodium lighten myocardial damage of exhaustive swimming exercise in some degree. |
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