The Significance And The Expression In The Laryngopharynx Carcinoma Of IGF-1R And U-PA

Objective:This research subject investigates, through immunohistichemistry SP method, the expressions of IGF-1R protein and u-PA protein in the tissue of laryngopharynx carcinoma and in the tissue adjacent to cancer, so as to discusses its mechanism of pathogenesis, development, invasion and metastasis, to find an useful indicator for early diagnosis of laryngopharynx carcinoma and pxovide the new methods and ideas for therapy and predict the prognosis of laryngopharynx carcinoma.Materials and methods:By adopting immunohistichemistry SP method, the expressions of IGF-1R protein and u-PA protein in the tissue of 55 laryngopharynx carcinoma cases, 42 adjacent to cancer cases and 40 epiglottis cyst cases were detected. All the datas were analyzed by SPSS 11.0 statistical software. The comparison of positive rates uses the Chi-square and Fisher, Statistically significant level was considered as "alpha equals 0.05".Results:①The rates of positive expression of IGF-1R protein in the tissue of laryngopharynx carcinoma, in the tissue adjacent to cancer and in the tissue of epiglottis cyst are 81.82%, 52.38% and 17.50% respectively, the difference between any two group is significant (P<0.01); The rates of powerful positive expression of IGF-1R protein in the positive expression are 72.72%, 30.95% and 2.50% respectively, the difference between any two is significant(P<0.01).②The rates of positive expression of u-PA protein in the tissue of laryngopharynx carcinoma, in the tissue adjacent to cancer and in the tissue of epiglottis cyst are 85.45%, 42.86% and 20.00% respectively,the difference between any two group is significant (P<0.01); The rates of powerful positive expression of u-PA protein in the tissue of laryngopharynx carcinoma, in the tissue adjacent to cancer and in the tissue of epiglottis cyst are 76.36%, 23.81% and 2.50% respectively, the difference between any two group is significant(P<0.01).③The rates of powerful positive expressions of IGF-1R protein in the tissue of metastasis and non-metastasis are 82.61% and 22.22% respectively. The difference in the two groups has statistical significance(P<0.01).④The rates of powerful positive expressions of u-PA protein in the tissue of metastasis and non-metastasis are 78.26% and 44.44% respectively. The difference in the two groups has statistical significance(P<0.01). ⑤The rates of positive expressions of IGF-1R protein in the groups of the 3-year survival and 3-year death are 76.92% and 88.89% respectively. The difference in the two groups has no statistical significance(P>0.05).⑥The rates of positive expressions of u-PA protein in the groups of the 3-year survival and 3-year death are 84.62% and 88.89% respectively. The difference in the two groups has no statistical significance(P>0.05).⑦The rates of positive expressions of IGF-1R protein in the groups of the 5-year survival and 5-year death are 75.00% and 86.67% respectively. The difference in the two groups has no statistical significance(P>0.05).⑧The rates of positive expressions of u-PA protein in the groups of the 5-year survival and 5-year death are 75.00% and 80.00% respectively. The difference in the two groups has no statistical significance(P>0.05).⑨There are 42 positive expressions of u-PA protein cases in the 45 positive expressions of IGF-1R protein cases. There are 5 negative expressions of u-PA protein cases in the 10 negative expressions of IGF-1R protein cases. The IGF-1R expression was closely correlated with the u-PA expression(P<0.01).Conclusions: 1. The rates of positive expressions of IGF-1R protein and u-PA protein are obviously increase in the tissue of laryngopharynx carcinoma, and in the tissue adjacent to cancer are higher than in the tissue of epiglottis cyst, . It is pointed out that IGF-1R and u-PA take part in the pathogenesis and development of laryngopharynx carcinoma.2.The powerful positive expressions of IGF-1R protein and u-PA protein obviously concern the lymph node metastasis. It indicated that the detection of IGF-1R and u-PA in the tissue of laryngopharynx carcinoma might be helpful to estimate the lymphatic metastasis of laryngopharynx carcinoma.3.The IGF-1R expression was closely correlated with the u-PA expression,which suggested that IGF-1R and u-PA played a positive role to each other in course of pathogenesis, development, invasion and metastasis of laryngopharynx carcinoma.