Regulation Of Shive-stopping Capsule On Expression Of DA, SOD, MDA And TH In Brain Of PD' Rats

Objective:To study the regulation of shive-stopping capsule on expression of monoamine oxidase B (MAO-B) and tyrosine Hydroxylase(TH ) in brain .Methods:The Parkinsonism model was prapaered by injection of 1% 6-OHDA in rat brain for two weeks. The tests were completed in 3 deffrent groups(Parkinsonism model group, normal controlled group and shive-stopping capsule treated group). The gene expression levels of MAO-B and TH in experemental rat brain were determined using RT-PCR. Moreover, the effect of shive-stopping capsule on activity of MAO-B in rat brain was determined by biochemical method.Results:In comparing to PD model group, it showed that shive-stopping capsule could obviously inhibit the increase of the gene expression level in rat brain. It was 4.728times lower than that in PD model group. Furthermore, it showed that the effects of shive-stopping capsule on activity of MAO-B is in accordance with mRNA level detected by RT-PCT, and the inhibitary rate of shive-stopping capsule on activity of MAO-B was over 40 percent. On the contrary,the gene level of TH was obviously increased in rat brain which were treated with shive-stopping capsule. In comparing to PD model group, it was 2.195times higher than that in PD model group.Conclusion:Shive-stopping capsule played the positive regulatory roles at the level of TH mRNA expressions in PD brain, and a negotive roles at the gene expression and activity of MAO-B.So it was suggested that shive-stopping capsule can be used to treat the PD in future.