Studies On The Expression Of Human Leukocyte Antigen-G (HLA-G) In Maternal Serum And Placentas In Preeclampsia

Objective:To investigate the expression of human leukocyte antigen-G(HLA-G) in maternal serum and placenta in pregnants with preeclampsia.Methods:The level of placenta HLA-G mRNA was measured by RT-PCR in 20' normal pregnants and 41 patients with preeclampsia(20 mild,21 severe);The serum sHLA-G concentration was measured by ELISA.Results.1.The level of HLA-G mRNA of placenta in mild and severe group(0.402±0.104,0.329±0.09 ) were significant lower than that in normal pregnant group(0.628±0.117)(P＜0.01).There was no significant difference between mild and severe group (P＞0.05).2.The maternal serum concentration of sHLA-G in mild and severe group (45.5±11.9units/ml,31.2±10.3units/ml) were significant lower than that in normal pregnant group(105.7±12.5units/ml)(P＜0.01).There was no significant difference between mild and severe group(P＞0.05).3.There was no correlation between the sersum concentration of sHLA-G or the level of HLA-GmRNA and the birth weight.There was no significance between early developed group and late developed group.4.There was a positive correlation between the level of HLA-G mRNA and the concentration of sHLA-G in the same pregnant(r=0.702,P＜0.01).Conclusions:1.The level of HLA-GmRNA in placenta and the concentration of sHLA-G in serum are all decreased in the pregants with preeclampsia.2.The sHLA-G concentration of sersum and the level of HLA-G mRNA of placenta in preeclampsia did not obviously correlate with the birth weight.There was no obviously significance between early developed group and late developed group.3.There was a positive correlation between serum sHLA-G concentration and HLA-GmRNA level in the same patient.