The Effects Of Qi-tonifying And Stasis-eliminating Therapy On Expressions Of TSP-1,TSP-2 And Their Receptor CD36 MRNA In Intracerebral Hemorrhagic Rat Brains

Background and Object:Intrcerebral Hemorrhage(ICH)is a common clinical severe disease,belonging to the category of stroke in Traditional Chinese Medicine.It has high incidence,death and Mutilation rate.It brings heavy burden to society.Since the view of Qixu Xueyu posed by a famous doctor of Qing dynasty,Wang Qingren,who initially invented the Buyang Huanwu Decoction(补阳还五汤),Qi-tonifying and stasis-eliminating(QTSE)therapy has been widely used and brought much benefit to patients in clinic.The modern foundation researchs have proved QTSE therapy can improve the blood flow in the brain hemorrhage zone and rectify the abnormal blood rheology changes in brain.However,the capillary vessel system surrounding the hematoma was damaged because of hemorrhage.Only through the reconstruction of capillary vessel system in the focal zone,the perfusion in the hematoma zone would be guaranteed,the microenvironment of the focus would be improved and the repair of nervous system would be accelerated.The angiogenesis is regulated both by promotive and inhibitive factors,thrombospondin typeⅠrepeats sequence(TSR)of thrombospondins-1(TSP-1)and TSP-2 can combine with its receptor CD36 to.inhibit the angiogenesis so protecting the blood vessel from accrementition rather too thick and pruning the neoformative tubiform through its accommodation to extracellular matrix.Therefore,we suppose that QTSE therapy could regulate the expressions of TSP-1,TSP-2 and CD36 mRNA in intracerebral hemorrhagic rats,which may promote the recovery of injured tissues through the formation,distribution and maturation of newborn vessels.In this research we try to study the mechanism of QTSE therapy through the oberservation to expressions of TSP-1,TSP-2 and CD36 mRNA in intracerebral hemorrhagic rats.Methods:155 Sprague-Dawley rats were randomly divided into six groups:including normal control group(n=5),sham control group(n=30), ICH model control group(n=30),Qi-tonifying(QT)-treated group(n=30), stasis-eliminating(SE)-treated group(n=30)and QTSE-treated group (n=30).By injecting collagenase typeⅦstereotaxically in to right globus, ICH model was made.Tales doses of normal sodium were injected in sham control group.QT,SE and QTSE-treated group were respectively administered at Buyang Huan wu decoction,QT component,SE component.Normal control group drunk freely,sham control group and ICH model control group were administered at tales doses of distilled water.The expressions of TSP-1,TSP-2 and CD36 mRNA yeas assayed by reverse transcription-polymerase chain reaction(RT-PCR)at day 1,4,7,14,21 and 28 after the onset.Results:No notably signals were detected in sham operated group and normal control group.TSP-1 mRNA peaked at 4 day(P＜0.01); TSP-2 mRNA peaked at 14 day(P＜0.01);while CD36mRNA peaked at 4 day and 21 day(P＜0.01).The expressions of TSP-1 mRNA in QTSE,QT,SE-treated groups were lower than that of ICH model group(P＜0.05);The expression of TSP-1 mRNA in QTSE therapy group is notably higher than that of ICH model group and QT-treated group at 4 day(P＜0.01);TSP-2 mRNA peaked at 14 day and its expressions in QTSE therapy group and SE-threated group are higher than ICH model group and QT-threated group;The expression of CD36 mRNA is diphase,it is lower during 1-4 days in QTSE therapy group and SE-threated group,but higher since 7th day.Conclusion:QTSE therapy could regulate the expressions of TSP-1,TSP-2 and CD36 mRNA in intracerebral hemorrhagic rats, which may promote the recovery of injured tissues through the formation,distribution and maturation of newborn vessels.