Detection Of Glucocorticoid And It's Receptor In The Patient Of Primary Hepatic Carcinoma And Correlative Factors Study

Investigation background:Heptocellular carcinoma is one of common malignant tumors in our country, which seriously jeopardize people's health. So far, there has been almost no effectual therapeutic drug except to adopt various of operative measures yet. It has intimate relation between glucocorticoid and tumour, the previons research reports that glucocorticoid can inhibit proliferation and induce apoptosis of hepatoma carcinoma cell, inhibiting proliferation and inducing cell differentiation of human ovarian cancer. Besides, glucocorticoid may be involved in inhibiting proliferation on human breat cancer cell lines cell, and inhibit the growth of vascular endothelial factor of tumour cell. Glucocorticoid receptor is important to signal conduction of tumour cell and the related gene of tumour and apoptosis, so it has been paid great attention the relation between GR and tumour. Ho,et al. observed that GR is discovered in the cytoplasm of majority primary hepatic carcinoma patient, which is the independently prognosis factor after primary hepatic carcinoma carcinosectomy. the survival rate of GR masculine is lower than GR negative. It is found that the GR content in tumor of liver is different. Most writer find that the GR content is descend in patient of cirrhosis of liver, while the GR content is increased in the hepatic cellular cancer. We need make a deep study on the relationship between GC and its receptor and the treatment and prognosis in primary hepatic carcinoma.Objective: To investigate the variation and mechanisms of effect on the glucocorticoid and it's glucocorticoid receptor (GR) of primary hepatic carcinoma patients.Methods:1. there are two group, one is consisted of 79 people who are primary hepatic carcinoma patients and the other group is consisted of 66 healthy people. We phlebotomize at six in the morning, then we separate blood serum and detect the glucocorticoid level by radioimmunity method.2. They are devided into primary hepatic carcinoma patients group and healthy people group, each group contains 22 persons. We phlebotomize at six in the morning, and detect the GR content with flow cytometry and observe the change of the glucocorticoid receptor (GR) content in primary hepatic carcinoma patients with 13 people after traditional Chinese drug combined therapy in our department.Results:1 .The glucocorticoid in primary hepatic carcinoma patients is lower by(P<0.05) than in the healthy people, the glucocorticoid is related to potassium, serum hepatitis and creatinine,extremely largest inverse correlation with the serum hepatitis, the interrelated coefficient is—44.484. the glucocorticoid is related to creatinine, gender and ALT in healthy adult, the glucocorticoid is lower in the female people than in the male people, the interrelated coefficient is—62.747.the glucocorticoid is elevated after treatment of the traditional Chinese medicine in primary hepatic carcinoma patients (P<0.01).2. The GR in primary hepatic carcinoma patients is lower by(P<0.01) than in healthy people. The GR combining power fluorescence in primary hepatic carcinoma patients is higher by(P<0.01) than in the health adult. The GR in primary hepatic carcinoma patients is elevated after treatment by(P<0.01).There is no discrepancy of GR combining power in primary hepatic carcinoma patients before and after treatment (P>0.5).Conclusion:1. The glucocorticoid in primary hepatic carcinoma patients is decreased and serum hepatitis is possiblly an important factor for glucocorticoid decreasing.2. The GR of primary hepatic carcinoma patients is lower than that in the healthy people, and the possible reason maybe that (1) cirrhosis of liver, (2) hepatic carcinoma lead to the damage of liver function, (3) the GR combining power is elevated in primary hepatic carcinoma patients, and the reason maybe that the GR decreasing lead to the GR combining power heighting.3. The GC and its receptor of primary hepatic carcinoma is changed obviously after combined therapy of traditional Chinese medicine. It reveals that influencing the GC and its receptor maybe an important effective target of curative effect of traditional Chinese medicine. But conclusive therapeutic action need be confirmed by detecting to correlated index of patient and following visiting duration and quality of life.Prospect:We initially detect the GC, its receptor primary hepatic carcinoma and GR combining power in this reseach and analysize the correlated patho-factors in multiple regression analysis, providing initially experimental data for exploring the relation between GR loss, invasion and therapy in primary hepatic carcinoma patients. In post-work, we will continue to flup and observe the duration and living quality et al indexs of the patient, approach the relationship between GC and its receptor level and development of hepatoma, and study the meaning of therapeutic action and prophylaxis relapse after applicating GC.