Disscussion Of The Relevance Between The Cortisol And Cortisol Rccptor In Hippocampus

Objective:To observe the differance of behaviors and level of cortisol in the blood in rats that are taken in chronic stress,while measure the expression of glucocorticoid receptor and mineralocorticoids receptor in hippocampus,thus to explore the dysfunction of Hypothalamus-Pituitary-adrenal (HPA) axis in depression in old age.Methods:Designated older male wister rats from Shandong Uiversity SPF animal center and divided stochasticlly into the normal control group (NC) and chronic unexpected stress group (CUS),NC accepts normally bred,absolute diet,water deprivation,immobilization,day and night inversion and so an are used for CUS randomly for3weeks.After the models succeeded, the blood was collected and level of cortisol was detected,while imrnunohistochemistry for GR and MR in hippocampus were made.Utilizing SPSS18.0to analyze all collected data.Results:1.The body weight gained,24h total intake,cleanupactivities, the consumption of1%sucrose intake,the score of open field in CUS group and NC group remarkably reduced,the rat model of depression has been established successfully on21st day.2.The level of cortisol in blood was more significantly increased in CUS than that in NC,P<0.01.3.The positive number of GR expression in hippocampus neuron,especially the area of CA3,decreased obviously in CUS,the area of DG was normal,while the positive number of MR expression in hippocampus neuron was no significant changes in CUS and NC.Conclusion:The dysfunction of HPA axis in older depression disolder was resulted in chronic unexpected stress,the level of cortisol in the blood elevated,hippocampus damage,especially in CA3,and the notably decrease number of GR.The number of MR expression in hippocampus neuron was normal.The dysfunction of HPA axis play an important role in elder depression.