The Research Of Physical Factors Infulence On Isoflavones Antioxygen Activity And Biological Efficacy

The soybean isoflavones belongs to the flavonoid isoflavones ingredient, as soon as is in the soybean growth process forms orders by type lives the metabolite. Recent years research indicated that the soybean isoflavones has many kinds of biology activeness and the health care function, therefore, the people to the soybean isoflavones attention degree distinct enhancement, its market prospect are also very attractive. However present, domestic and foreign mainly concentrates in view of the soybean isoflavones research in its extraction and the content determination aspect, at present was still rare in view of its active research report.The paper has mainly studied the soybean isoflavones activeness determination one new method opposition flowing injection chemiluminescence law, has determined its determination method: The main creeping motion pump will carry the class alcohol 30% and Luminol 0.03mmol·L^-1 (pH9.2 carbonate buffer solution) the input analysis system. After treating the baseline steadily, vice-creeping motion pump 5 10-4 mol·L^-1K₃Fe(CN)₆ solution through 16 introduction valves (note type time: 10s) pours into carries in the class, with its mix, the speed of flow is 2.0mL·min-1. Take K₃Fe(CN)₆-Luminol-30% ethyl alcohol as the blank illumination system, the luminous intensity is I0, the sample enhancement signal is, takes peak valueΔI= IS - I0 to carry on the quota. The luminous intensity counts the expression.The experiment has determined the influence soybean isoflavones active physics condition: Under the liquid condition, is suitable the preserved density for 10μg/mL, the suitable temperature for -20℃, may the long time (≤24d) preserve, but has not been suitable for the long time preservation. The solid state may the long time preservation, the ferruginous vessel maintain to the soybean isoflavones activeness advantageous, the long time illumination urges the isoflavones activeness weaken.The paper respectively take the black abdomen fruit fly and 3 soybean varieties (DongNong 42, DongNong 46, DongNong 1215) as materials, whether there is compared with the active different density's soybean isoflavones to the fruit fly each physiology target's influence, and processed after oxidant H2O2 the soybean seed's germination percentage and the bud length and the soybean isoflavones active relations. The result indicated that activated to fruit fly life, , depress MDA content, enhance SOD and CAT activeness, but the non-antioxygen activity soybean isoflavones does not have the same phenomenon. Soybean isoflavones activeness cuts the germination percentage to drop, bud long shortening, proved that soybean isoflavones activeness and seed's vigor has the relevance.The findings have provided the valuable reference for the soybean isoflavones reasonable effective use, has certain theory significance and the practical application value.