The Research Of New Method In Sanitary Inspection Based On The Chemiluminescence

Chemiluminescence （CL） detection has become quite a useful tool oftrace analysis due to its simplicity, low cost and high sensitivity. In recentyears, luminescent reagents and systems have been improved and perfected.The development of new system and the conjunction with other techniques,capillary electrophoresis and flow injection based on chemiluminescence havebeen widely used for its character of fast, simple and sensitive.In this study, chemiluminescence detection was combined with flowinjection method to determinate the papaverine hydrochloride injection andluminal-Ag（III） chemiluminescence system was combined with capillaryelectrophoresis separation technology for the determination of1-and2-naphthol in urine and arginine in human serum.PART1Determination of Papaverine Hydrochloride in PapaverineHydrochloride Injection with Flow-Injection Chemiluminescence DetectionObjective: To establish a novel flow injection chemiluminescencemethod for Papaverine Hydrochloride.Methods: Papaverine hydrochloride was found to strongly enhance theCL signal of the luminol-K₃Fe （CN）₆system under the alkaline medium, andbetween a certain range, the concentration of papaverine hydrochloride wasdirectly proportional to the CL signal. Based on that phenomenon, a newFI-CL method was developed for the determination of papaverinehydrochloride, and parameters affecting the CL intensity were optimizedsystematically.Results: With the optimized conditions, the corresponding linearregression equation was established over the range from5.00to200.00μg/mL（r=0.999）, the detection limit was1.70μg/mL and the average recovery was 101.30%.Conclusions: The proposed method has been satisfactorily applied forthe determination of papaverine hydrochloride injection, and the result wasaccurate and reliable. PART2Determination of1-and2-naphthol in human urine by capillaryelectrophoresis with chemiluminescence detectionObjective: To establish a new method for determination of1-and2-naphthol by capillary electrophoresis with chemiluminescence detection.Methods: It was found that1-and2-naphthol could inhibit the intensityof the luminal-Ag（III） chemiluminescence system in alkaline medium. Usingthis character, a rapid and sensitive method for determination of1-and2-naphthol was developed with capillary electrophoresis coupled withchemiluminescence detection. The experimental conditions were optimizedand drawing the standard curve, then the urine sample was determinated afterprocessing.Results: With the optimal conditions, limit of detection for1-and2-naphthol were2.00×10-7and6.00×10-6mol/L, the relative standarddeviations were2.03%-4.16%and the average recoveries were respectively101.50%and99.30%.Conclusion: The proposed method was successfully applied to theanalysis of human urine, the result was satisfactory. PART3Determination of arginine in human serum by capillary electrophoresis with chemiluminescence detectionObjective: To establish a new method for determination of arginine bycapillary electrophoresis with chemiluminescence detection.Methods: It was found that arginine could enhance the intensity of theluminal-Ag（III） chemiluminescence system in alkaline medium. Using thischaracter, a rapid method for determination of arginine was developed withcapillary electrophoresis coupled with chemiluminescence detection. Theexperimental conditions were optimized and drawing the standard curve, thenthe serum samples were determinated after processing.Results: With the optimal conditions, limit of detection for arginine were2.50×10^-5mol/L, the relative standard deviations was1.75%and the recoverywas96.83%.Conclusion: The method was simple and fast and was successfullyapplied to the analysis of human urine.