| Objective: This study is to control beeswax and other related products quality and to provide experimental data for developing used by checking the fingerprints of beeswaxes.Methods: (1) Select ten beeswaxes in different sources nationwide. According to the method of the People's Republic of China professional standard- beeswax SB/T 10190-93, we determined the physico-chemical properties of these beeswaxes, i.e., density, melting point, refractive index, acid value, iodine value, and saponification number. (2) Chromatographic conditions were as following: capillary column gas chromatography, SE-54,30m,0.32mm×0.25m. Temperature programming method was applied. Temperature of the column was from 50℃to 200℃at the rate of 5℃.min-1, and then from 200℃to 295℃at the rate of 5℃.min-1, kept 26 minutes, and total time was 60 minutes. The injection port temperature was 250℃, FID temperature was 280℃. Supporting gas was highly pure nitrogen and at the rate of 40ml.min-1, hydrogen gas and air were all at the rates of 50ml.min-1, split ratio was 50:1. The beeswaxes fingerprints of ten different nationwide sources were determined by using gas chromatography and their common peaks were found. According to《Technical requirement of the fingerprint in injection of Chinese materia medica(tentative standard)》, we calculated relative retention time, relative peaks area, the percentage of non-common peaks area, relative percentage content of their common peaks ,and calculated the similar degree of beeswaxes from different sources.Results:(1) The results of determination of the physico-chemical property of beeswaxes from 10 nationwide sources were significant different; however, they were all consistent with the requirement of People's Republic of China professional standard-beeswax SB/T 10190-93. We have showed that the beeswaxes we purchased were qualified products. They can be used as samples for determining beeswax fingerprint. (2) Among the beeswaxes fingerprints of ten nationwide sources, we obtained seventeen comparatively steady common peaks except the control. The relative retention times were of little variable, their RSD were small than 1%. Relative peaks area were large different, the largest difference was between Henan and Hebei sources, the third common peak area was of 40 times difference. The percentage of non-common peaks area was lower than 10%. Different content were determined in different sources beeswaxes. The content of the eighth common peak composition were the largest different, the composition content of Dalian source was 30.791%, Guangdong source was 43.489% and Hainan source was 52.831%. This composition content of other sources was also large. The result showed that this composition was the essential component of beeswax. We calculated the similar degree according to the Dalian source, which was used for reference. The similar degrees of Shanghai and Hebei sources were low but other sources were high. The sources of Guangdong, Henan, Shandong had the best similar degree, separately for 0.953, 0.967, and 0.919. It showed that the compositions and therapeutic effect between Dalian and the three sources had a good concordance.Conclusions:(1) In the determination of beeswaxes fingerprints of ten different nationwide sources. We found that they were all consistent with《Technical requirement of the fingerprint in injection of Chinese materia medica(tentative standard)》. And they can be used for beeswax fingerprint. (2) The chemical ingredients of beeswaxes from different source were much similar and little different. As the basic ingredient of Skin Growth Promoting Ointment, beeswax was selected from Dalian source. We control beeswax source strictly from fingerprint. By this way, we suggest that Skin Growth Promoting Ointment has invariable,definite and persistent therapeutic effect.(3) The fingerprint we got from ten different sources have good stability and validity, they can be used as a method for strict quality control of beeswax source. From identification of their chemical ingredient, we have provided information those different sources beeswaxes were of different therapeutic effect.
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