Research On Efficacy Of Effective Parts Combination Rule Of Exocarpium

Objective:By adopting modern analyzing techniques and drug effect comparision, studying combination rules of the efficiency parts such as volatile oil,total flavone,total polysaccharides, etc. By analyzing integrately, we expound the scientific meaning of exocarpium's effect on easing pain and resisting inflammation,turing phlegrn and stoping asthma, anti-oxygenation, which can afford experimental gists for revealing material bases of efficacy and proving synergism the main effective parts and components.Mothod:Using steam distillation method to extract the volatile oil and solvent mothod to extract total flavone and polysaccharide components; Using Spectrophotometry to determine the content of totle flavone and amylose, the RP-HPLC method to mensurate content of naringin , using ammonia causing rat cough method,injecting hydroxybenzene red to rats' trachea,making rats wresting mothed,heating broad method, making rats' ear swelling method to study the drug effect and search the feasible effect parts and their combination of exocarpium;we also introduce the equality designing method to optimize the most effective part or their combination and look for the combination rules.Result:1. research on exocarpium's effect partsSeparating the volatile oil, total flavone and amylose and other components (such as fatty acid and coumarin, etc)from chemical components of exocarpium. Determine the content of total flavone and amylose , the total flavone is calculateed by naringin, not fewer than 80%; use the phenol-vitriol method to calculate the glucose, the part which contains more than 75% is the determined as the main effective part.2. Studies on eliminating phlegm and relieving asthma , antitussive effect of effective parts and combination of exocarpium Compared with the blank team, studies on eliminating phlegm and relieving asthma,antitussive effect of each effective part show that every effective part has statistic significance and it presents certain dose-effect relationship. As to eliminating phlegm effect, the high dose group of volatile oil is the best;and it is also the best team in relieving cough. Using uniform design to study effects of effective parts combination, the results shows:As to the eliminating phlegm and relieving asthma effect, using the equation theory to choose the best combination, the high dose of volatile oil team, which is accord with the single-dose investigation result. By analyzing the statistic process, compatibility of volatile oil,total flavone and total polysaccharides can enhance effect. Compared with traditional water decoction, there is significant distinction with volatile oil team;We choose the better combinatiom group 11(combination 11 is composed of all high-dose of exocarpium, the equare is 0.8:40:80:20) by analyzing statistics and compare with traditional water decoction, there is significant distinction of combination ll;Compared with high-dose team of volatile oil team, combination 11 has no significant distinction.. As to the antitussive effect, volatile oil and total flavone play main roles. Based on the data, we choose combinatiom 11, compared with traditional water decoction, there is no significant distinction with group 11.3. Studies on analgesic and anti-inflammatory effect of effective parts and combination of exocarpiumUsing hot plate test and twisting method to determine analgesic effect of each effective part of exocarpium, the result is that compared with blank group, each experiment team have certain analgesic effect, among them . The combinatiom 11 has the significant effect. Using uniform design to study combination of every effective part, the best combination is high-dose of total flavone. In the hot plate test, compared with traditional water decoction, high-dose of total flavone group have statistic significance 30 minites after taken drugs;other periods have no significant distinction;The better combinatiom 11, compared with water decoction, has no significant difference. In the twising method, compared with water decoction, total flavone and combination 11 of high-dose team have no significant difference. With xylene inducing rats' ears swelling method, and albumen inducing rats' toes swelling method, we determine anti-inflammatory effect of each effective part of exocarpium,the result shows that compared with blank group, each team has significant distinction, and the most significant team is combination 4 and 6;compared with traditional water decoction, both have no significant difference.4. Studies on antioxidation of effective parts and combination of exocarpiumComparing antioxidation of every effective parts of exocarpium by pyrogallol atoxidation method and o-Phenanthroline method vitroly, comparesion of inhibition rate shows that every sample has certain oxidation resistance. Single factor investigation in pyrogallol atoxidation method,the high-dose of total flavone team has the most significant effect. Single factor investigation in o-Phenanthroline method, high-dose of other teams have better effect. Studying effective parts combination by uniform design , with pyrogallol atoxidation method, we choose high-dose of total flavone team, by analyzing inhibition rate, group 11 has the most significant effect, all are higher than the traditional water decoction team;with o-Phenanthroline method, we choose the high-dose of total flavone team, analyzingΔA, group 4 (combination 4 is composed of low-dose of volatile oil,tolal flavone of high-dose,high-dose of total polysaccharide,middle-dose of other contents) have the significant effect, but antioxidation of the regular water decoction is the best.5. Studies on pharmacodynamics of traditional water decoctionDrugs are used in clinic mostly by water decoction form, and make the effect of preventing and treating diseases. The research compare traditional water decoction group with other experimental teams, it shows that it has certain eliminating phlegm and relieving asthma,antitussive effect; it has certain analgesic and anti-inflammatory effect; it has strong antioxidation.Conclusion:1. Studies on effective parts of exocarpiumBy content determination controlling quality of drugs, which ensure homogeneity of quality and efficacy.2. Studies on eliminating phlegm and relieving asthma,antitussive effect of effective parts and combination of exocarpiumAs to eliminating phlegm and antitussive effect areconcerned, the high-dose of volatile oil group has the most instinctive effect in every investigation. Of the combination, the group 11 is the most instinctive,Compared with traditional water decoction, combination 11 and high-dose of volatile oil team both have instinctive distinction in eliminating phlegm efficacy experiment; in antitussive experiment, Compared with traditional water decoction, there is no statistic signification.3. Studies on analgesic and anti-inflammatory effect of effective parts and combination of exocarpiumAs to the analgesic and anti-inflammatory effect, each effective part has certain efficacy, but the high-dose total flavone group is the most effective group. the study of combination shows that in the analgesic effect experiment, combination 11 are most effective, but compared with water decoction, it has no significant difference; in the anti-inflammatory effect experiment, the super combination is the high-dose of total flavone group and total polysaccharide, compared with water decoction, the two teams have no significant distinction.4. Studies on antioxidation of effective parts and combination of exocarpiumComparing the antioxidation effect of each effective part exocarpium by pyrogallol method and phenanthroline method show that each sample have certain antioxidation. In pyrogallol method, high-dose of total flavone group have the most instinctive effct;in phenanthroline method, high-dose of other team are most instinctive. Combination study shows that in pyrogallol method, we choose the high-dose of total flavone team, by analyzing inhibition rate, group 11 is the most instinctive, and it is better than water decoction; by phenanthroline method, we choose the high-dose of total flavone team, analyzingΔA, group 4 (the proportion of combination 4 is, volatile oil,tolal flavone,total polysaccharide other contents, equare0.2:40:80:10) is most distinctive, but as to the antioxidation, the water decoction is most effective.5. Studies on pharmacodynamics of traditional water decoctionTraditional water decoction may have some rationality,but because of morevolatile oil is lost than others, the on eliminating phlegm and relieving asthmaeffect is not so good as other groups; it has certain antitussive effect andanalgesic and anti-inflammatory effect; the most instinctive effect is inantioxidation.