Relationship Among HPV Infection, Deletion Of E6TP1 And Rap1GAP Genes Exons In Cervical Disease

Objective: To reveal the molecular mechanism of cervical cancer by exploring the relationship among deletion of E6TP1, Rap1GAP genes exons and HPV infection in cervical disease.Methods: Cervical exfoliated cells from 200 cases of normal and cervical disease were detected for HPV infection by Hybrid Capture 2 assay; 85 cases were chosen according to HPV results, 16 cases with normal, 16 cases with cervicitis, 30 cases with CIN and 23 cases with cervical cancer. Deletion of E6TP1 exon 6, Rap1GAP exon 11 and Rap1GAP exon 19 were identified by polymerase chain reaction(PCR) amplification.Results:1. Infection rate of HPV was 19.23%(10/52) in normal, 20%(13/65)in cervicitis, 41.67%(25/60) in CIN, 52.17%(12/23) in cervical cancer.2. Deletion rate of Rap1GAP E11 was 0 in normal, 6.25% in cervicitis and 43.33% in CIN, 43.48% in cervical cancer; 44.19% in HPV positive group and 11.90% in HPV negative group of all cases. Deletion rate of Rap1GAP E11 in CIN or in cervical cancer was significantly higher than in normal or cervicitis (P < 0.05). Deletion rate of Rap1GAP E11 was significantly higher in HPV positive group than in HPV negative group (P<0.05). Deletion rate of Rap1GAP E11 was positively correlated with HPV infection and the degree of cervical lesion (P<0.01).3. Deletion rate of Rap1GAP E19 was 0 in normal, 12.5% in cervicitis, 26.67% in CIN and 34.78% in cervical cancer; 34.88% in HPV positive group and 7.14% in HPV negative group of all cases. Deletion rate of Rap1GAP E19 in CIN and cervical cancer was significantly higher than in normal (P<0.05). Deletion rate of Rap1GAP E19 was significantly higher in HPV positive group than in HPV negative group (P<0.05). Deletion of Rap1GAP E19 was positively correlated with HPV infection and the degree of cervical lesion (P<0.01).4. There was no deletion of E6TP1 E6 in all cases.Conclusions:1. There was positive correlation between HPV infection and deletion rates of Rap1GAP E11 and E19 exons.2. The degree of cervical lesion was positively correlated with deletion rates of Rap1GAP E11 and E19 exons.3. HPV infection and cervical disease didn't accompany with deletion of E6TP1 E6 exon.