Effects Of Electric Acupuncture With Acupoints Of Yangming Meridians On The Expression Of PAC-1 And CD62p In ACI Patients

Background: Acute cerebral infarction refers to a serious disease which is threaten to the people' s life . The sequela, such as hemiplegia, anepia, etc. seriously affected the quality of life of patients. In recent years, how to promote the recovery of limb function of Stroke patients with hemiplegia is being the focus of the study in the world. Platelet activation has a closed relationship with thrombogenesis in Stroke patients. Large number of studies have shown that the electric acupuncture treatment with acupoints of yangming meridians is an effective way to treat the ACI. However, there is no clinic report on the effect of electric acupuncture on the expression of PAC-1, CD62p and D-Dimer in ACI patients.Objective: To observe the effects of electric acupuncture with acupoints of yangming meridians on the expression of PAC-1, CD62p and D-Dimer as well as the neurological grade with the method of Barthel index, NIH Stroke scale and simplify Fugl-Meyer. Approach for the relevance of severity of Pathogenetic condition with PAC-1,CD62p and D-Dimer and try to find a new theoretical proof for the electric acupuncture treatment in ACI.Methods: 58 cases of ACI patients, admitted to the Foshan Hospital of TCM from November 2007 to April 2008, confirmed by CT or MRI, were randomly divided into electric acupuncture group and control group. The conventional therapy were used in each group, and the electric acupuncture treatment was added in electric acupuncture group on the basis of conventional therapy. The expression of PAC-1,CD62p and D-Dimer in 58 cases of ACI were respectively detect by flow cytometer, etc. And the neurology grade and recovery of lower limb function was evaluated with the method of Barthel index, NIH Stroke scale and simplify Fugl-Meyer. 20 cases of healthy people were choosen to have a contras with ACI patients. Results: In the acute stage, the expression of PAC-1 and CD62p in ACI patients, which is in the relevance of Pathogenetic condition severity, significantly increased than healthy people (P<0.05, P<0. 05) . The expression of CD62p in each group markedly decreased than its own prior treatment on the 14th day, being significantly different(P<0. 05, P<0. 05). However, the expression of CD62p after post-treatment has no difference between two groups (P>0. 05) . Contrast to the control group, the expression of PAC-1 in electric acupuncture group markedly decreased on 14th day (P<0.01) , but there was no significant difference between prior-and post-treatment in control group. The expression of D-Dimer in each group increased on the 14th day. In comparison with control group, the increasing of D-Dimer in electric acupuncture group was significant lower (P<0. 05) . The advance of neurology grades, which was evaluated with the method of Barthel index, NIH Stroke scale and simplify Fugl-Meyer in each group, have marked difference (P<0. 5) between electric acupuncture group and control group on the 14th day after treatment. Compared with control group, Fugl-Meyer's grade was improved on the 14th day but no difference in Barthel index, NIH Stroke scale. Both PAC-1 and CD62p have a significant relevance with severity of Pathogenetic condition (P<0. 05, P<0. 01) .Conclusions: Platelet activation has been existing in the acute stage of ACI. PAC-1 and CD62p have a closed relationship with ACI. Both of them have a significant relevance with severity of Pathogenetic condition. Electric acupuncture treatment with acupoints of yangming meridians in the early stage can make recovery of paralyzed limbs and reduce expression of PAC-1 in order to diminish thrombogenesis.