The Study Of Main Pharmacodynamics And Mechanism Of Action Interfere In IBS Of The Capsule Of Changji' An

1 The objectsThe capsule of Changji' an is composed of many Chinese herb which called Chinese medicine,it has a good effect for the spleen the sparse liver, the refrigeration dispel the effect which wet, the astringent intestines stop flow swiftly(has special Chinese medical meaning), especially treats diarrhea IBS(Irritable bowel syndrome), the chronic colitis and so on. This medicine has been tested through clinical usage and observation by the first affiliated hospital of Guangzhou University of Chinese medicine more than 20 years. It proves that the capsule of Changji' an has the function that treats diarrhea IBS indeedly .The research is to provide the capsule of Changji' an to meet the clinical, study of requirements which the national standards of 6 th of new medicines include. the research of mechanism of the capsule of Changji' an is to expound that this preparation treats IBS the function mechanism and develops this medicine for the depth research results.2 The methodsThis research is mainly divided two parts. the first part is the literature search including the domestic and foreign literature as the foundation of the research . The second part is the experimental study, including: Main pharmacodynamics research, acute toxicity experimental study, function mechanism research. (1) by the Hot pan (using the mouse as experiment animal) testing the function of relieving pain of the capsule of Changji' an, Value the influence on the model which caused by Fanxieye , a Chinese herb, the experiment of mouse small intestine propelling movement, the experiment of mouse abdominal cavity blood capillary permeability carries on the intestines to the capsule of Changji' an treatment diarrhea IBS pharmacodynamics research. (2) secure appraisal by the test of toxicity , the test of maximum volume of the endurece through filling mouse stomach without dead . (3) through inspecting the change of gastrointestinal hormone CGRP and SP, it can be found the mechanism of the capsule of Changji' an intervents diarrhea IBS .3 Results3.1 Literature search resultThe literature search indicated that regarding IBS, the modern medicine curative effect is often unsatisfied, and the poisonous side effect is big. The Chinese medicine gained experience richly by several thousand year . compared two medicine in treating the IBS , it concluded tha the Chinese medicine has its superiority, because its curative effect be proofed firmly and its side effect is small, and it has the reseach value in future .3. 2 Experimental study result(1) pharmacodynamics experimental indicated that the capsule of Changji' an be able to enhance the threshold value of Kunming mouse feeling painful obviously, its pain-relieving' s effect strong and the weak, presents certain quantity effect relations tendency. and it Can reduce the times that diarrhea model Kunming mouse' s bowel move and the quantities of black excrement of the model. Its function is consistent with the clinical observation; it also can weaken the small intestine creeping motion movement; The anti-inflammatory experiment indicated that has the obvious anti- inflammatory function. (2) the acute toxicity experiment: through filling the mouse stomach by the most large dose which is equl to 500 times of a adult dosage everyday . through observating animal body weight change, after each group of mice for medicine, has not presented the obvious toxicity to respond, the mouse spirit has the dispirited slightly, the hair is normal, the activity reduces, the body weight has not reduced, the biggest dosage presents one to die. After for medicine, altogether observes 7d, the first day appears dies 2, the 2nd day later has not seen the animal death, the survival mouse state of mind has not seen exceptionally, is unable to determine the median lethal doses (LD50) of the capsule of Changji' an . from the experiment it can conclude tha the Chinese medicine which called capsule of Changji' an can be used security, mouse endures the quantity for the medicine is 22. 2g/kg most greatly, is equal in adult date oral administration dosage 250 times (adult body weight according to the 50kg idea).(3) through mechanism experiment it found that: (10×200 time) observes CGRP, SP immunity fiber distribution in the colon mucous membrane with the optical microscope made in Beijing University of Aeronautics and Astronautics medicine pathology image analysis system, in computation stochastic 4 field of vision colon mucous membrane CGRP, SP fiber average OD value, on behalf of CGRP, SP quantity, the OD value is separately bigger, indicated that CGRP and SP are more. CGRP, the SP fiber can be seen in various experimental groups colon mucous membrane, its distribution has not seen the significance change; For the medicine group, the blank group and the model group compare, three groups of distributions also do not have the difference. In three group of between colon mucous membrane' s CGRP, SP fiber number (root /100μm×100μm) do not have the difference. In the model group colon mucous membrane CGRP, SP the average OD value distinction normal group (P value <0. 05). The normal group compared with capsule of Changji' an low, middle and high group , medicine group and BuPiYiChangWan pill group , there is non-statistics meaning. 4 Research conclusionThe reseach including pharmacodynamics experiment, the acute toxicity test as well as the intervention diarrhea IBS mechanism indicated that the capsule of Changji' an have the functions of well pain-relieving, to stop flow swiftly , to anti - inflammatory and so on . It show tha the capsule of Chuangji' an is accordance with the modern medicine function of r the spleening the sparse liver , the refrigeration dispels, the astringent intestines to stop wet flows swiftly . this Chinese medicine has the merit of the rang of safe is big. the test result prompts it in the normal oral administration dosage situation, is small to the organism poisonous side effect, conforms to the new medicine development highly effective, low poisonous, the safe request. This research mechanism part inspects the target is not yet comprehensive, but must the further research..