Studies On Pharmacognostical And Chemical Constitue Nts Of Sambucus Adnata

Sambucus adnata Wall is belonged to family Caprofoliaceae. It has been used as a folk herbal drug in China. All parts of this plant can be used medically. It is pungent and sweet in flavour, warm in property, has the effects of dispelling wind and wetness, promoting meridian flow, invigorate the circulation of blood, alleviating water retention. It is mainly used for the treatments of acute and chronic nephritis, the pain of rheumatic, pruritus by rubella, sequela of poliomyelitis, fracture and so on.In the recent years, the researches about chemical components and their activities of pharmacological on the plants of family Caprofoliaceae are becoming deeper and deeper. However, the studies on Sambucus adnata Wall have been reported rarely.This paper includes two chapters. The first chapter describes the characters of Sambucus adnata Wall. 4 parts including root, stem, leaf and leafstalk of this plant's microstructure are observed and described, and we identify their mainly chemical components primarily. In the second chapter, chemical investigation of Sambucus adnata Wall has been undertaken. And the structures of compounds we isolated were analysed. In these studies, the plants we used were herborized from E'mei Mountains. We hope to find new compounds through the different producing areas of this plant. This research will not only promote chemical development of natural products, but also provide theories for developing our country's traditional medicinal plants. Furthermore, our results could help to exploit our province's natural plant resource.In study on pharmacognosy of this plant, we summarized characteristics and microstructures of Sambucus adnata Wall different from other plants, and proposaled ursolic acid will become the chemical indentifying character of this plant. In the study of chemical constituents, by the differences in polarity among compounds, we isolated pure compounds on silica gel chromatography and preparative thin layer chromatography. Then their structures were analysed based on spectral datum.Finally, 11 compounds have been isolated from Sambucus adnata Wall. Nine compound's structures have been identified through NMR and ESIMS. They are 5, 7, 3', 4'-Tetramethoxyflavone-3- O- Rhamnopyranosyl-(1→6)- Glucopyranoside 1, 1-(3-hydroxy-4-methoxyphenyl)-1', 2'-Ethanediol 2, ursolic acid 3, 1-(3, 4, 5-Trimet-hoxyphenyl)-1', 2'-Ethanediol 4, lariciresinol 5,β-sitosterol 6, Stigmasterol 7, oleanolic acid 8 and 3-oxo-oleanolic acid 9. The compounds 1, 2, 4, 5, 6, 7 and 9 were obtained from this plant for the first time.