The Expressions Of PDGF And Their Relations To Angiogenesis In Endometrial Cancer

ObjectivesEndometrial cancer(EC) is one of three malicious tumors of femals genital tract. In recent years,it's morbidity has a trend to increased. It threatens women's lives seriously. Studying its mechanism in tumogenesis and seeking for effective method in therapy have become important problem for study. Researches have indicated that the growth of tumor is dependent on angiogenesis. Tumor cells can select many angiogenic factors that promote tumors' growth. So, antiangiogneic therapy has become very important in tumor therapy. Microvessel density(MVD) is quantitative indicator of angiogenesis. Researches have indicated that platelet-derived endothelial growth factor(PDGF) play an important role in angiogenesis of rumors. But a few researches about them in EC is reported in foreign journals and scarcely is reported in Chinese journals. The present study was to investigate the expressions of PDGF and their relation to angiogenesis in endometrial cancer(EC) so as to learn the mechanism of tumogenesis in EC and to offer the theoretic basis to the antiangiogenic therapy of endometrial cancer.MethodsEndometrial cancer specimens were obtained from 40 patients who were surgically treated from 2002.1 to 2006.12 in the Shengjing Hospital of China Medical University.A control group with normal endometrium (20 cases) and atypical hyperplasia of endometrium(20cases).All specimens were stained immunohisto-chemically for CD₃₄ and PDGF identified immunohistochemically with specific antibody . Use of anti-CD₃₄ antibody marked vascular endothelial cells to count MVD. Observed slices under 400 double light microscope,photoed under digital camera, input fields random seleted into MetaMorph,converted staining intensity of cells to quantification index, calculated opital density .All experimental data were analysed with t test and processed by SPSS 13.0.There was a statistics significance when P<0.05.Results1,There were significantly differences of MVD in normal endometrium,atypical hyperplasia of endometrium and endometrial cancer (P<0.05). It's highest in endometrial cancer and lowest in normal endometrium. There were significantly differences of MVD in well differentiated,moderately differentiated and poorly differentiated (P<0.05). It's highest in poorly differentiated and lowest in well differentiated. MVD was gradually higher following with muscular infiltrated.2,PDGF was significantly higher in endometrial cancer than in normal endometrium and atypical hyperplasia of endometrium(p<0.05).There were no significantly difference between normal endometrium and atypical hyperplasia of endometrium(p>0.05). There were no significantly difference between moderately differentiated and poorly differentiated(p>0.05).It was lower in well differentiated than in moderately and poorly differentiated (p<0.05). In which with muscular infiltrated it was higher than in normal endometrium(p<0.05).3,Spearman correlation analysis was used to express the relationship between MVD and PDGF, r=0.848, (p=0.000<0.05).They were positive correlation.Conclusions1,MVD was significantly higher in EC than in normal endometrium and with the development of EC MVD increased which indicated angiogenesis may play an important role in development of EC .MVD may be a valuable indicator in prognosis judgement of EC.2,The expression level of PDGF in EC is significantly higher than in normal endometrium, which indicated they may play a role in the tumogenesis of EC. The expression of PDGF were no significantly difference between moderately differentiated and poorly differentiated. And it didn't concerned with muscular infiltrated. We can illustrate that it may produce an effect in earlier stage of EC.3,MVD and PDGF were positive correlation, we can illustrate that PDGF may produce an effect in angiogenesis...