| Objective: Endometrial cancer is one of the common gynecologicalmalignancies, and the morbidity and mortality shows gradual upward trend inrecent years. Abnormal proliferation of tumor cells and surrounding tissueinvasion and metastasis is an important mechanism of tumor genesis anddevelopment, therefore accurately determine the tumor stage and riskassessment, and reasonable treatment is the premise to improve efficacy,improved quality of life and prognosis.Minichromosome maintenance proteins (Minichromosome MaintenanceProteins, MCMs) first found in yeast, is a significant association of thehomologous series of a class of close family of proteins. With the effectivepreservation of gene sequences, similarities have been found in Xenopus, miceand human tissue. MCM2protein and other family members formed beforecopying complex adhesion to the DNA site formed by replication originrecognition complex (origin recognition complexb ORC) and the cellproliferation cycle factor6(cell division cycle, Cdc6) protein. This compoundas a "pass" of DNA replication is indispensable for DNA replication in alleukaryotic cells[1-5]. Compared with normal tissues, tumor cells has a fasterproliferation, aberrant regulation of DNA replication is the most important linkin the process of abnormal cell proliferation,and MCM proteins are importantregulatory proteins in the process of DNA replication in all eukaryotic cells. Invitro studies have shown that these proteins can be detected in the wholeprocess of cell replication, however, disappears in the differentiation andresting, which makes them to be specific markers of cell replication. Therefore,the antibodies of the protein to become a valuable cell proliferation detection[6].This topic is designed on the basis of original research, we detected the expression of MCM2protein in surgical resection specimens of endometrialcarcinoma by immunohistochemical staining method.Compared with normalendometrial tissue and combined with clinical data and postoperativeimmunohistochemistry of results, we analysised the expression of MCM2protein in endometrial carcinoma ocurrment and development and comparedthe expression of MCM2protein with that of estrogen receptor (Estrogenreceptor, ER), progesterone receptor (Progesterone receptor, PR).There for,wemay provide new tumor markers to judge the degree of malignancy andaccurate assessment of prognosis in endometrial cancer.Methods: Endometrial cancer tissue samples were collected from53cases who underwent surgical resection and confirmed by pathologically (after1year follow-up, including22cases patients with recurrence,11cases died ofthe disease in22patients and concurrent control patients without recurrence31cases).53cases of histological types of cancer are the Palace of endometrialadenocarcinoma, this study does not include the special histological types oftumors such as clear cell carcinoma or serous carcinoma. All patients beforesurgery without radiotherapy, chemotherapy, Chinese medicine and hormonetherapy, record the patient name, age, tumor differentiation, pathologic stage(according to the2009latest FIGO stage), lymph node metastasis,postoperative1-year follow-up and the results of immunohistochemical resultsand clinical data, and normal endometrial tissue specimens were selected form20cases who underwent hysterectomy for leiomyomas or cervicalintraepithelial neoplasia in the same period. We investigated the expression ofMCM2protein using immunohistochemical SP staining method specimens.Applied statistical software SPSS for the windows13.0, we analysised theexpression of MCM2protein, the ER and PR in endometrial adenocarcinomaand the correlation between MCM2protein with ER, PR by χ2test and thespearman rank correlation method.Results:1. Detect the expression of MCM2protein in endometrial cancer tissuesand normal endometrial tissue by immunohistochemical SP method:the expression of this protein in endometrial cancer and normal proliferativeendometrium tissues was no significant difference (P>0.05), endometrialcancer and normal proliferative endometrium is more extensive than theprotein in the normal secretory phase endometrium, the staining intensity wassignificantly increased, with significant differences (P<0.01). The expressionof it has nothing to do with age.2. The53cases of endometrial cancer patients with ER and PRimmunohistochemistry results showed that: the expression of ER, PR inpoorly differentiated endometrial cancer tissue was significantly lower than inwell-differentiated endometrial carcinoma, the difference was statisticallysignificance (P<0.05), the expression of ER, PR in pathologic stage III~IV ofcancer tissue was significantly lower than stage Ⅰ~Ⅱ cancer tissue, thedifference was statistically significant (P<0.05). Compare with the recurrenceafter1year, without recurrence of cancer tissue has more expression of ERand PR, the difference was statistically significant (P<0.05). The expression ofER, PR in endometrial carcinoma has nothing to do with patient age, lymphnode metastasis.3. ER, PR positive expression rate was61.5%,57.7%in endometrialcarcinoma with positive staining of MCM2protein, ER, PR positiveexpression rate of endometrial carcinoma with MCM2protein negativeexpression was100%.Conclusions:1. MCM2protein is highly expressed in endometrial carcinoma, and areclosely related with tumor stage, histological grade, lymph node metastasis,postoperative1-year follow-up results, and there was no significant correlationwith age. The expression of MCM2protein in normal proliferativeendometrium tissue also is significantly increased.2. The expression of ER, PR in endometrial carcinoma associated withtumor stage,degree of differentiation,after1year follow-up results lymphnode metastasis, and there was no significant correlation with age.3. The expression of MCM2protein and ER, PR in endometrial carcinoma there was no significant correlation; but all of which aresignificantly correlated with the prognosis of endometrial cancer.4. Detection MCM2protein and ER and PR of endometrial cancer mayhave some significance in high-risk screening and prognostic evaluation. |
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