Research On Ahotoactive Activity And Pharmacognosy Of Bulgaria Inquinans

Bulgaria inquinans is a kind of ascomycete that Belongs to Bulgariaceae. It is a kind of special development potential fungi that to be used of food and medicine concurrently, it has the anti-tumor action, the photoactive activity, reduceing red blood cell rate of sedimentation of blood stasis rat, anti-sudden change and so on. The ingredient content of nutrition is very high. It mainly distributes in northeast area of our country, and this fungi often appear in the aphotic place of the coal mine. Now domestic enterprise has developed new medicine with Bulgaria inquinans treating psoriasis, this fungi also were massively purchased by Japanese from China, the bag material cultivation and the big area generalization needs to further investigation, that will provides the evidence for factitial cultivation and to gain raw material. Along with thorough research of the light power therapy, it should be clear about the photoactive activity constituent and the empoison mechanism especially, to formulate quality specification for the corresponding raw material.Pharmacognostic research of Bulgaria inquinans displayed that characteristic trait, microscopic trait, physichemical trait and molecular biology trait in this paper, The results showed that fruit bodies were snuff color and dotted with yellowish brown pits at the beginning, and then their upper side split into one or several pores, at last the surface of body turned black and crimpled. The asci are clubbed in shape, the ectal excipulum is made up of circular cells with hairs on the outside which contains chromophore-like substance, the medullary excipulum is consisted of interlaced mycelia imbedded in gelatine. The saffron yellow mycelia and septatus achromatous mycelia with growth clubbed conidium were gained by ascospore discharging method. The mycelia obtained by fruiting body tissue method were hyaline and filamentous, some mycelia composed of circular cell became bead-like, dark brown or yellowish. Comparing the DNA sequences from fruiting body and mycelia gain from liquid culture using strain of tissue culture, their similarity is 99.65%. After comparing the DNA sequences from fruiting body and AY789345 in GenBank, their similarity is 99.80%. The showing of organization separation obtains mushroom Spawn is stable and reliable, it is the pure mushroom spawn of Bulgaria inquinans.Fruiting body and fermental fruiting body of Bulgaria inquinans were extracted and separated in this article, 5 compounds and 1 component with photoactive activity were obtained in all, expertised 4 compounds, they are aremalonic acid, hexadecanoic acid, 4, 8-nonadecane dienoic acid and 2, 3-consecutive naphthyl-4-(1-hydroxy-normai-butyl)-5-valerolactone respectively. Posterior 3 compounds were separated from Bulgaria inquinans for the first time, 2, 3-consecutive naphthyl-4-(1-hydroxy-normal-butyl)-5- Valerolactone is a new compound. Photoactive activity component was predicated perylenequinones composition preliminarily.The Q component which obtained from the petroleum ether extract layer was used the GC-MS associated analysis, it mainly retrieved to 22 compounds. The G component which obtained from the chloroform extract layer was used the GC-MS associated analysis, it mainly retrieved to 7 compounds. They are mainly a kind of small polarity fatty group hydrocarbon and the fatty acid.The extract of acetone, extract of chloroform coming from extract of acetone and Y4 component and Y5 component which separated from the extract of chloroform displayed photoactive activity in photoactive activity experiments. Ear index displayed significant deviation to extract of acetone intragastric administration by 1000mg/kg for illumination series, ear index and skin index displayed extreme significant deviation to extract of acetone intragastric administration by 2000mg/kg for illumination series. Ear index and skin Index displayed extreme significant deviation to extract of chloroform intragastric administration by 260mg/kg and 520mg/kg for illumination series. Ear index displayed significant deviation to Y4 component intragastric administration by 200mg/kg for illumination series, ear index displayed extreme significant deviation to Y5 component intragastric administration by 200mg/kg and 100mg/kg for illumination series, skin Index displayed extreme significant deviation to Y4 component intragastric administration by 200mg/kg and Y5 component intragastric administration by 200mg/kg and 100mg/kg for illumination series. There is Certain dose-effect relationship in the photoactive activity and the dosage, its photoactive toxic attack also has certain dormiens period. Extract of acetone was used in rats through intragastric administration by 1g/kg for 5 days, illumination 2 hour every day, ears of rat started to fester, claws also became quite purple at fourth day, ears was disappeared by fester at sixth day. Photoactive activity of Bulgaria inquinans is also checked by experiment of rat. The locus that is sensitive to light is damaged acutely.