Effect Of Bixieqianggupian On Experimental Osteoporosis In Rat

The object: To research the effects of Bixieqianggupian(BXQBP) on osteoporosis in rats.Methods and results: The experimental models of osteoporosis (OP) induced by ovariectomy(OVX), retinoic acid(RA) and dexamethasone(DXM) in rats were introduced in this study. In the same time, the influence on tibia fracture healing in rats was also observed. Moreover, the anti-inflammation effects and analgesia of BXQBP in mice and rats were also studied.The body weight gain induced by OVX was prevented obviously by administering BXQBP. And serum estradiol and bone gla protein (BGP) were examined by RIA, and results showed that estradiol increased and BGP decreased. Bone mineral density (BMD), bone mineral content (BMC) and bone mass of femur had increased, moreover, calcium Content of bone (monitored by atomic absorption) had been improved significantly after BXQBP administration. Furthermore, biomechanical characters of bone were measured by three point bending test, and the anti-bend intensity and maximum bend strength increased remarkably. The alkaline phosphatese (ALP) decreased. And amount of urine calcium (Ca) and hydroxyproline (HOP) decreased obviously. However, effect on the proliferation of endometria was not obvious.The RA induced OP model: The methods of examine were applied according to that of above mentioned. Compared with model, the BMD and BMC increased markedly in OP rats (i.g. 30days). And bone mass and calcium content had increased. Then BGP and ALP decreased by administering BXQBP. The anti-band intensity and maximum bend strength increased evidently. And ejection of urine Ca and HOP decreased obviously. The bone trabecula became thinner, and arranged in disorder in OP rats, however, the status was reversed obviously by administrating BXQBP.The OP model also induced by DXM in rats: Effect against weight losing caused by DXM was observed in groups of three doses (i.g. 12 weeks). And BMD, BMC, bone mass and calcium content increased evidently. The results showed that the fracture healing had been enhanced obviously at three doses (i.g.40 days), callus growth was promoted and bone rigidity was reinforced. Moreover, BXQBP had the anti-inflammation and analgesia effects in mice and rats.Conclusion: BXQBP was showed an anti-osteoporosis action in rats.