Study On The Relationship Between Peri-implantitis And Suspected Pathogens

Objective:1. To detect the colonization of 7 subgingival suspected pathogens in partialedentulous patients with and without peri-implantitis.2. To investigate the relationship of periodontal suspected pathogens, peri-implantitisand the bone loss.3. To investigate the microbiologic pathogeny of peri-implantitis, and to find the mainpathogen of peri-implantitis in the early stage.4. To find a method to diagnosis peri-implantitis with early prevention and treatment.Methods:1. 88 patients with 89 implants were divided into 3 groups: 1) peri-implantitis group,29 peri-implantitis-patients with 29 implants 2) peri-mucositis group, 29peri-mucositis-patients with 30 implants 3) healthy group, 30 healthy patients with30 implants by random.2. Peri-implant pocket depth (PPD) and oral pan tomography was recorded as clinicalparameters.3. Buccal subgingival plaque was collected from mesial to distal by same dentist withnon-bacterial carbonous fibred tool.4. DNA of subgingival plaque was distilled.5. Colonization of 7 periodontal suspected pathogens was detected by polymerasechain reaction (PCR): A. actinomyceremcomitans (A. a),P. intermedia (P. i),P.gingivalis(P. g),Treponema denticola (T. d),P nigrescens(P. n),Campylobacterrectus (C. r),B. forsythus (B. f).Results:1. Prevalence of these 3 groups was different.(1) Higher detection of B.f,P.i and T.d was found in peri-mucositis group than in healthy group. Higher detection of P.g,B.f,T.d and P.n was found inperi-implantitis group than in healthy group. Higher detection of P.n,P.g and T.dwas found in peri-implantitis group than in peri-mucositis group. There issignificant difference among these 3 groups (P＜0.05).(2) No significant difference existed among these 3 groups in detections of A.a andC.r (P＞0.05).2. Logistic analyze: P.g,T.d and P.n played an important role in peri-implantitis. (OR=5.830, 61.037 and3.672).Conclusion:1. P.g,P.i,P.n,B.f and T.d are major bacterium that relate with soft and hardtissue around the implant, especially with peri-implantitis. P.g cooperated with P.nand T.d, might cause bone loss around implant. When the detection of P.i,T.dand B.f increase, peri-implant pocket depth has deepened trend; The furtherreproduction of pathogens of peri-mucositis may be the reason for peri-implantitis.2. Suspected pathogens of peri-implantitis were detected by using 16SrRNA basedPCR, which could be helpful with early diagnosis, prevention and cure ofperi-implantitis.