| Peri-implantitis refers to inflammation of tissues surrounding dental implants. It is one of the main causes of implant failure, due to subsequent loss of surporting bone around implant with peri-implantitis. As inflammation in peri-implantitis is caused by a complex interaction between host's immune system and pathogen (mostly bacteria), it is very important to understand the immunological background of peri-implantitis, and this will be favorable to diagnosis and therapy of the disease. The aim of present study was to investigate the expression of cytokines and inflammatory cell phenotypes in the tissues surrounding implant with peri-implantitis. The results are as follows:1. Sandwich enzyme-linked immunosorbent assays(ELISA)was used to examine the cytokines in peri-implantscrevicular fluid (PICF). It showed that interleukin-1 β(IL-1β ), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF- α ) could be detected in PICF from the patientswith peri-implantitis. The detected levels of IL-1 β , IL-6 and TNF-α were 2.3275 ± 0.3283ng/ml, 5.7123 ± 0.7620ng/ml and 21.1780±2.5073ng/ml respectively.2. Immunochemistry staining showed that T-cells(CD3+ cells) were the predominant inflammatory cells in the connective tissues surrounding the implants. Among these, CD4+ cells were the most numerous subgroup. (CD14+) were observed as scattered cells. B-... |
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