Expressions And Correlations Of MT And MT-3 In Esophageal Squamous Cell Carcinoma

Objective: The genetic and environmental factors areinvolved in the development of carcinoma. We detected theexpression of metallothionein (MT) gene and metallothionein-3(MT-3) gene in the tumor tissues and corresponding normalesophageal mucous membrane of esophageal squamous cellcarcinoma (ESCC). In this study, we investigate the correlationbetween expression of MT and MT-3 and clinical pathologicalfeatures.Methods: Immunohistochemistry staining SP was used todetect the expression level of MT and MT-3 in 60 tumor tissuesand corresponding normal mucous memebrane of ESCCSubsequently, we analyze the relationship between postop-erative pathological fetures, such as differentiation,lymphanode transferation and TNM stage, and expression ofMT and MT-3.Results: 1 The pattern of MT expression is obviouslydifferent between ESCC and their corresponding incisal degenormal tissue. The distribution of MT staining in incisal edgenormal tissue is even, and distribution of positive cell also even.Positive staining is obviously in plasm. The distribution of MTstaining in tumor tissue is even, and distribution of positive cell also even. Positive staining is obviously stronger in plasm thanthat in nucleus.2 Expression level and ratio of MT were significantly higher intumor tissue than that in incisal edge normal tissue and tissuewith innocent esophageal disease. There was statisticalsignificance among them (P＜0.05) which show positivecorrelaltion (r=0.657, P＜0.05).3 The relationship between clinical pathological features andMT expression in tumor tissues:3.1 There was statistical significance among well, moderatelyand poorly differentiated ESCC (p＜0.05) and with thedegradation of differentiation, the expression of MT wasincreased. We got positive correlation result betweendifferentiatin and expression (r=0.526, p＜0.05).3.2 There was statistical significance between MT expressionlevel and TNM stage of tumor (p＜0.05) and with thedegradation of TNM stage, the MT expressin was increased.We got positive correlation result between them (r=0.515,p＜0.05).3.3 MT expression level was obviously lower in tumor tissueswithout lymphanode transferation than that with lymphanodetransferation and there was statistical significance (p＜0.05).3.4 There was no significance difference bewteen expressionlevel or ration and clinical factors, such as sex, age, length ordepth of tumor and typing.4 The relationship between clinical pathological features and MT-3 expression in tumor tissues:4.1 There was statistical significance among well, moderatelyand poorly differentiated ESCC (p＜0.05) and with thedegradation of differentiation, the expression of MT-3 wasdecreased. We got negative correlation result betweendifferentiatin and expression (r=-0.475, p＜0.05).4.2 There was statistical significance between MT-3 expressionlevel and TNM stage of tumor (p＜0.05) and with thedegradation of TNM stage, the MT-3 expressin was decreased.We got negative correlation result between them(r=-0.388,p＜0.05).4.3 MT-3 expression level was obviously higher in tumortissues without lymphanode transferation than that withlymphanode transferation and there was statistical significance(p＜0.05).4.4 There was no significance difference bewteen expressionlevel or ration and clinical factors, such as sex, age, length ordepth of tumor and typing.Conclusions: 1 Compared with incisal dege normal tissue,the MT expression modality was different in ESCC tissue whichsuggests important significance of MT distribution in tumor cell.2 The expression level and ratio of MT in ESCC tissue wasobviously higher than that in normal tissue.3 MT correlates with clinicalpathological features in ESCC andthe increasing MT expression had a postive correlation withpooly differentiation, advanced degree and lymphonode transferation.4 Compared with incisal dege normal tissue, the MT-3expression modality was different in ESCC tissue whichsuggests important significance of MT distribution in tumor cell.5 The expression level and ratio of MT in ESCC tissue wasobviously lower than that in normal tissue.6 MT correlates with clinicalpathological features in ESCC andthe decreasing MT-3 expression had a negative correlation withpooly differentiation, advanced degree and lymphonodetransferation.7 Expression of MT and MT-3 was different in ESCC. The roleof MT-3 differed from MT in the development of ESCC.