Study About The Expression And The Correlation Of CIPAa And HPU16-e7in ESCC

Objection1、To detect and analysis the expression of CIP2A and HPV16-E7in ESCC andnormal esophageal epithelial mucosa.2、To study the relationship between CIP2A and HPV16-E7in esophageal squamouscell carcinoma tissue; meanwhile to elucidate the possible molecular mechanisms that HPVinfection induced carcinogenesis of ESCC.3、To explore the relationship between the two indications and clinical pathologicalcharacteristics, as well as the significance in the development of esophageal squamous cellcarcinoma.MethodsUsing immunohistochemical SP staining, we detected the expression of HPV16-E7and CIP2A in the esophageal squamous cell cancer tissue which came from50cases ofpatients, compared with the adjacent normal epithelium. Meanwhile the clinical factorswere statistically analyzed.Results1、In the esophageal squamous cell carcinoma tissues and the normal mucousmembrane of esophagus, the positive expression rates of HPV16-E7and CIP2A weresignificant different (P≤0.05), which prompted HPV16-E7and CIP2A express inesophageal squamous cell carcinoma specifically.2、There were no significant differences of the HPV16-E7and CIP2A expressionswith tumor grades of the esophageal squamous cell carcinoma（P≥0.05）, which promptedboth of them had no significant correlation with the differentiation degree of esophagealsquamous cell carcinoma.3、The HPV16-E7positive rate of smoking patients (84.6%,22/26) was significantlyhigher than that of the non-smoking patients (54.2%,13/24). 4、The positive rate of HPV16-E7and CIP2A protein have no significant correlationwith age, gender, degree of differentiation, lymph node metastasis and clinical stage.5、The CIP2A protein positive expression rate of HPV16-E7-positive group was91.4%(32/35), the CIP2A protein positive expression rate of HPV16-E7-negative groupwas60.0%(9/15), there was statistically significant difference between two of them (P≤0.05). The results suggested that the expression of HPV16-E7may directly or indirectlyaffected the expression and activity of protein CIP2A.ConclusionIn this thesis, we mainly studied the oncoprotein E7of HPV16and a new discoveredoncoprotein CIP2A in esophageal squamous cell carcinoma, including the relationship ofHPV16major oncoprotein E7and CIP2A as well as their correlation with clinicalparameters.The study results showed that there was no or low expression of HPV16-E7andCIP2A in normal esophageal mucosa tissue, meanwhile the expression of them was high inesophageal squamous cell carcinoma; The expression of HPV16-E7was significantlycorrelated with smoking, which indicated that smoking may contribute to HPV infection;The expression of HPV16-E7protein and CIP2A protein have no significant correlationwith age, gender, tumor depth of invasion, histological grade, degree of differentiation,lymph node metastasis and clinical stage, suggesting that although both of them canpromote the development of esophageal squamous cell carcinoma, but can not yet judgethe degree of malignancy and stage of progress in esophageal squamous carcinoma of asbiological cell indicators; There is a correlation between HPV16-E7and CIP2A, whichmatchs the literature that claimed HPV oncoprotein can upregulate the expression level ofthe CIP2A prompting there may also existed a similar molecular biological mechanisms inesophageal squamous cell carcinoma, but to verify these mechanisms needs furtherresearch. We presumed that HPV may promote cancer through inhibiting the expression ofCIP2A, which is depended on the foundings in our experiment and the researches of others.This innovations of this paper has not been reported in the current relevant literature. It hasbeen confirmed that the CIP2A played an important role in the occurrence anddevelopment of esophageal squamous cell carcinoma. According to the results of this paper,we have gotten the IHC evidence to speculate that uncontrolled HPVE7expression canincrease the expression level of the CIP2A, which can promote the development of cellsfrom precancerous lesion to malignant tumors. Further study on this subject will reveal thenew regulatory pathway of HPV protein’s carcinogenic process clearly, which can provide a new way for the future to diagnose and therapy esophageal squamous cell carcinoma inclinic.