Expression And Clinical Significance Of Livin And HSP70 In Laryngeal Squamous Cell Carcinoma

Objective:The aim of this study is to detect the expressions of HSP70 and Livin,a novel inhibitor of apoptosis protein in laryngeal squamous cell carcinoma.To ascertain the clinical significance of HSP70 and Livin in laryngeal squamous cell carcinoma.Methods:The expressions of Livin and HSP70 were detected by the immunhistochemical S-P method in 28 specimens with precancerosis of larynx(26 specimens with papilloma of larynx and 2 specimens with karatosos of larynx),55 specimens with laryngeal carcinoma and 20 specimens with vocal cord polyps.Results:①The positive expression rates of HSP70 were respectively 78.2%,39.3%,5.0%in laryngeal carcinoma,precancerosis of larynx and vocal cord polyps.There was a significant difference of the expression rate of HSP70 between the laryngeal squamous cell carcinoma and the precancerosis of larynx(P＜0.05);There was a significant difference of the expression rate of HSP70 between the precancerosis of larynx and the vocal cord polyps(P＜0.05).②The positive percentage of HSP70 in laryngeal squamous cancer was significantly higher correlated with pathological grade and metastasis of lymph node(P＜0.05)but had no obvious correlations with clinical stage and age(P＞0.05).③Livin protein were expressed in 37 of 55 LSCC cases.Of the 18 cases were not expressing livin.It was expressed in 6 of 28 precancerosis of larynx,and none of 20 vocal cord polyps.There was no difference of precancerosis of Livin between vocal cord polyps and precancerosis of larynx(P＞0.05).When compared with vocal cord polyps and precancerosis of larynx,laryngeal squamous carcinoma had significantly more frequencies of livin expression(P＜0.001).④The positive percentage of Livin in laryngeal squamous cancer was significantly higher correlated with metastasis of lymph node and clinical stage(P＜0.05)but had no obvious correlations with pathological grade,sex and age(P＞0.05).⑤There was a significant difference of the expression rate of Livin between the laryngeal squamous cell carcinoma with positive expression of HSP70 and that with negative expression of HSP70(P＜0.05),whose positive expression rates were 76.7%and 33.3%respectively.The positive expression of Livin in laryngeal squamous cell carcinoma with positive expression of HSP70 was significantly higher than that with negative expression of HSP70. Conclusion:①HSP70 may have an important role during the development of larynegeal papilloma;Expression of HSP70 can be used as important pathological grade.②The positive percentage of HSP70 in laryngeal squamous cell carcinoma was significantly higher correlated with pathological grade and metastasis of lymph node but had no obvious correlations with clinical stage and age.Detection of the expression of HSP70 may be useful in evalution of the aggressiveness of human laryngeal squamous cell carcinoma.③The up-regulated expression of Livin in laryngeal squamous cell carcinoma suggests that Livin plays an important role in tumor occurrence and progress through inhibiting cell apoptosis. Therefoe,we conclud that Livin may be the index for identifying LSCC.Livin can provide new appropriate target for gene therapy to LSCC.④The positive percentage of Livin in laryngeal squamous cancer was significantly higher correlated with metastasis of lymph node and clinical stage but had no obvious correlations with pathological grade,sex and age.⑤There exists relation between expression of HSP70 and Livin in laryngeal squamous cell carcinoma.They may be influence apoptosis though interacting.