| Objective: Laryngeal cancer accounts for about1%~5%of allmalignant tumors, the major pathological types for squamous cell carcinomaof larynx, accounting for about90%, the incidence rate has a trend ofincreasing in recent years. Cell disorder balance between proliferation andapoptosis which effects the occurrence and development of laryngealcarcinoma is one of the many factors. Apoptosis inhibiting protein (Livinprotein) has the cell apoptosis activity. Caspase-3protein is one of the key ofprotease mammals cell apoptosis. The cytoplasmic Smac gene can combineLivin gene BIR structure domain specific, thereby inhibiting its anti-apoptoticeffects, accelerated apoptosis. Smac genes and Caspase-3genes may play anegative regulatory role in Livin resistance of the genes of cell apoptosisactivity. This topic through the study of laryngeal carcinoma, paracarcinomaand normal laryngeal mucosa in Livin, Smac and Caspase-3expressionreveals Livin, Smac, and Caspase-3in laryngeal squamous cell carcinomadevelopment influence and possible mechanisms, as well as their expressioncorrelation, and extend the laryngeal cancer treatment approaches, and providethe theoretical basis.Method：1Applicating of immunohistochemistry (immunohistochemistry, IHC) S-Pmethod, we detect the expression of Livin, Smac and Caspase-3in38cases of laryngo-carcinoma,21cases of para-carcinoma and17cases ofnormal laryngeal mucosa.2Applicating of Flow Cytometrye (low cytometry, FCM) method, wedetect the expression of Livin, Smac and Caspase-3in38cases oflaryngo-carcinoma,21cases of para-carcinoma and17cases of normallaryngeal mucosa. 3Using SPSS13.0to analysis the statistical data of experiment, withP≤0.05for a statistically difference, P≤0.01indicates statisticallysignificant difference.Result：1The expression of Livin protein1.1IHC: Applicating of IHC S-P method, we detected the expression ofLivin in laryngo-carcinoma, para-carcinoma and normal laryngealmucosa, and its expression tendency was decreased, the three groupswere significantly different(P<0.01). A further comparison of Livinexpression, laryngo-carcinoma group was higher than para-carcinomagroup（P<0.01） and normal laryngeal mucosa group (P<0.01), nosignificant difference was observed between para-carcinoma group andnormal laryngeal mucosa group (P>0.05). It is confirmed that thepositive expression of Livin was correlation with clinical staging, lymphnode metastasis and pathological grading of tumor (P<0.05).It was notrelated with the clinical classification, tumor size, smoking history, ageand sex (P>0.05).1.2FCM: The quantitative expression of Livin protein (FI) inlaryngo-carcinoma, para-carcinoma and normal laryngeal mucosa, and itsexpression tendency was decreased, there was statistic significance(P<0.01). The quantitative expression of Livin in laryngo-carcinoma washigher than in para-carcinoma (P<0.01) and in normal laryngeal mucosa(P<0.01) respectively. No significant difference was observed betweenpara-carcinoma group and normal laryngeal mucosa group (P>0.05). It isconfirmed that the positive expression of Livin was correlation withclinical staging (P<0.05), lymph node metastasis (P<0.01) andpathological grading of tumor (P<0.05). It was not related with theclinical classification, tumor size, smoking history, age and sex (P>0.05).2The expression of Smac protein2.1IHC: Applicating of IHC S-P method, we detected the expression of Smac in laryngo-carcinoma, para-carcinoma and normal laryngealmucosa, and its expression tendency was increased, the three groupswere significantly different (P<0.01). A further comparison of Smacexpression, laryngo-carcinoma group was lower than para-carcinomagroup （P<0.05） and normal laryngeal mucosa group (P<0.05).Nosignificant difference was observed between para-carcinoma group andnormal laryngeal mucosa group (P>0.05). It is confirmed that thepositive expression of Smac was correlation with clinical staging(P<0.05), lymph node metastasis (P<0.01) and pathological grading oftumor (P<0.01), It was not related with the clinical classification, tumorsize, smoking history, age and sex (P>0.05).2.2FCM: The quantitative expression of Smac protein (FI) inlaryngo-carcinoma, para-carcinoma and normal laryngeal mucosa, and itsexpression tendency was increased, there was statistic significance (P <0.01). The quantitative expression of Smac in laryngo-carcinoma waslower than in para-carcinomaand in normal laryngeal mucosa (P<0.01,P<0.01)respectively No significant difference was observed betweenpara-carcinoma group and normal laryngeal mucosa group (P>0.05). It isconfirmed that the positive expression of Smac was correlation withclinical staging (P<0.05), lymph node metastasis (P<0.01) andpathological grading of tumor (P<0.05), It was not related with theclinical classification, tumor size, smoking history, age and sex (P>0.05).3The expression of Caspase-3protein3.1IHC: Applicating of IHC S-P method, we detected the expression ofCaspase-3in laryngo-carcinoma, para-carcinoma and normal laryngealmucosa, and its expression tendency was increased, the three groupswere significantly different (P<0.01). A further comparison of Caspase-3expression, laryngo-carcinoma group was lower than para-carcinomagroup（P<0.05）and normal laryngeal mucosa group (P<0.01). Nosignificant difference was observed between para-carcinoma group and normal laryngeal mucosa group (P>0.05). It is confirmed that thepositive expression of Caspase-3was correlation with clinical staging,lymph node metastasis and pathological grading of tumor(P<0.05). Itwas not related with the clinical classification, tumor size, smokinghistory, age and sex (P>0.05).3.2FCM: The quantitative expression of Caspase-3protein (FI) inlaryngo-carcinoma, para-carcinoma and normal laryngeal mucosa, and itsexpression tendency was increased, there was statistic significance(P<0.01). The quantitative expression of Caspase-3in laryngo-carcinomawas lower than in para-carcinomaand and in normal laryngeal mucosa(P<0.01,P<0.01）respectively, no significant difference was observedbetween para-carcinoma group and normal laryngeal mucosa group(P>0.05). It is confirmed that the positive expression of Caspase-3wascorrelation with clinical staging (P<0.01), lymph node metastasis(P<0.01) and pathological grading of tumor (P<0.05). It was not relatedwith the clinical classification, tumor size, smoking history, age and sex(P>0.05).4The correlation of Livin, Smac and Caspase-3in laryngeal carcinoma4.1Livin correlation with Smac: the quantitative expression of Livin protein(FI) and Smac protein (FI) in laryngeal carcinoma contents a linearnegative correlation, linear regression equation is Y=2.379-0.642X (r=-0.830,P=0.000).4.2Livin correlation with Caspase-3: the quantitative expression of Livinprotein (FI) and Caspase-3protein (FI) in laryngeal carcinoma contents alinear negative correlation, linear regression equation is Y=2.654-1.109X (r=-0.818,P=0.000).4.3Smac correlation with Caspase-3: the quantitative expression of Smacprotein (FI) and Caspase-3protein (FI) in laryngeal carcinoma presentsno correlation (r=0.262,P=0.113>0.05).Conclusion:1The expression of Livin in laryngo-carcinoma group was higher than para-carcinoma and normal laryngeal mucosa.2The expression of Smac and Caspase-3in laryngo-carcinoma group waslower than para-carcinoma and normal laryngeal mucosa.3The quantitative and qualitative expression of Livin in para-carcinoma andin normal laryngeal mucosa was lower than in laryngo-carcinoma.However, the quantitative and qualitative expression of Smac andCaspase-3in laryngo-carcinoma group was lower than para-carcinomaand normal laryngeal mucosa. It Suggest that high expression of Livinand low expression of Smac, Caspase-3may be associated with laryngealcarcinogenesis.4It is confirmed that the expression of Livin, Smac and Caspase-3proteinin laryngeal carcinoma was correlation with clinical staging, lymph nodemetastasis and pathological grading of tumor, but that was no correlationwith the clinical classification, tumor size, smoking history, age and sex.5In laryngeal carcinoma, the quantitative expression of Livin protein andSmac,Caspase-3protein contents linear negative correlationrespectively.There is no linear correlation between Smac and Caspase-3.It Suggest that three proteins exist between mutual adjustment,interaction. |
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