The Effects Of Mevalonate On Proliferation And Cell Cycle Phase Of Culured Human Glomerular Mesangial Cells

Objective:To study the effects and mechanism of mevalonate on proliferation and cell cycle phase of culured human glomerular mesangial cells(HMC)in vitro.Thus we can approach the method of suppressing proliferation of culured human glomerular mesangial cells(HMC)and offer the theory bases for stains pleiotropy.Methods:HMC was presented by China xiehe university.Cells were cultured by 1640 in 50ml culture flask with 37℃,5%CO₂.The first test was divided 8 groups:control group(10%FCS), MVA medication administration teams(1nmol/L,10nmol/L,100nmol/L,1μmol/L,0μmol/L,100μmol/L,1mmol/L).HMC proliferation was determined by MTT colormetric method at different concentrations of MVA at 48h and found 100nmol/L is the most evident dose.HMC proliferation was determined by the same method with 100nmol/L MVA at 12,24 and 48h.The seconed test was divided 4 groups:control group(10%FCS),MVA group(100nmol/L),LOV group(10um/LLOV),MVA intervention group(10um/LLOV+100nm/LMVA).HMC proliferation was determined by MTT colormetric method.By means of detection HMC A_490nmWith different doses of MVA,10um/LLOV and 100nm/LMVA intervention with enzyme odetection -meter to determine influence of different groups to HMC proliferation.The value of S/G₁ was determined by flow cytometry to oberse influence of 10um/LLOV and 100 nm/LMVA to cell cycles.Results:1 MVA was found to stimulate HMC proliferation within the range from 10 nmol/L to 100 nmol/L in a dose-dependent manner.The value of A₄₉₀nm increased from 0.408±0.019 in control group to 0.509±0.011 in maximal concentration(100 nmol/L of MVA).Compared with control group the experimental group(10,100 nmol/L of MVA)increased increased significantly (P＜0.05)2 The stimulatory proliferation on HMC by MVA at the concentration of 100 nmol/L was time-depented within 12-48 h.The values of A₄₉₀nm were individually 0.201±0.040,0.326±0.019and 0.509±0.011 at 12,24and 48 h.Compared with others group they are different significantly(P＜0.05).3 The values of A_{490 nm}were individually control group(0.296±0.015,0.489±0.011),MVA group(0.326±0.019,0.509±0.011),LOV group(0.108±0.010,0.216±0.009), MVA intervention group(0.290±0.017,0.490±0.013)at 24,48h.Compared with control group MVA and LOV group change significantly(P＜0.05),but MVA intervention group changes insignificantly(P＞0.05).4 The values of S/G₁ were individually control group(0.257±0.007,0.556±0.005),MVA group(0.356±0.001,0.643±0.002),LOV group(0.108±0.009,0.301±0.010),MVA intervention group(0.230±0.013,0.541±0.011).Compared with control group MVA and LOV group change significantly(P＜0.05),but MVA intervention group changes insignificantly (P＞0.05).Conclusions:MVA is a HMC proliferation and cell cycle phase stimulator.LOV can inhibit the HMC proliferation and induce the G₁/S transition arrest.Concomitant addition of 100nm/L/L mevalonic acid can reverse its effect.1 MVA was found to stimulate HMC proliferation within the range from 10 nmol/L to 100 nmol/L in a dose-dependent manner.Higher dose of MVA can inhibit the HMC proliferation. Perhaps it is concerned with cytotoxity.2 The Stimulatory proliferation on HMC by MVA at the concentration of 100 nmol/L was time-depented within 12-48 h.3 10um/L LOV can inhibit the HMC proliferation.Concomitant addition of 100nm/L mevalonic acid can reverse its effect.4 MVA is a cell cycle phase stimulator.10um/L LOV can induce the G₁/S transition arrest. Concomitant addition of 100nm/L mevalonic acid can reverse its effect.