Costumes, Bi Particles On The Cell Proliferation And Cell Cycle Of Ra Mesangial Proliferative Nephritis Cell Model

Objective:To culture and identify the glomerularmesangial cells(GMC) of rats.According to the MTT shade selection experiment and the flow cytometry to approach if wangbikeli(WBKL) serum can influent the cell's proliferation and cell cycle of the GMC which is stimulated by immune complex(IC).Methods:Culture GMC in vitro.The cellularmarkers including Mouse Anti-Desmin,cytokeratin and factorⅧwere detected with immunocytochemistry in order to identify GMC and draw the growth curve. At the same time prepare the rats' serum after give drugs.Passage the cell to 3-8 era,and subgroup,there are blank control group,model control group,blank serum group,WBKL serum low,middle,high dose groups,total glucosides of pae-ony(TGP) serum group,prednisone serum group.Add the serums to GMC to do the cytotoxic experiment.Exept the blank control group,add AIgG(aggregated IgG)to other groups,and give serum to the groups pro rate.48h later,do the MTT shade selection experiment,determine the optical density value(OD value).Then do the flow cytometry to observe the changes cell circle.Results:Cells in culture began to grow on day 4,then the cells appeared stellate or triangular with irregular cytoplasmic projections in about18 days observed through light microscope.The immunocytochemistry stainings of Anti-Desmin were positive in the cultured GMC,while those of factorⅧand cytokeratin negative.The doubling time of the sub-generation of GMC was 4 days.All kinds of serums have no cytotoxic effect.The OD value of model group is obviously higher than blank group(P＜0.001),and the OD value of WBKL10%,20%serum groups is lower than model group(P＜0.01-0.001), and higher the density lower the OD values,OD values of TGP serum group and prednisone serum group are both lower than model group(P＜0.001).In model group the MC's ratio in S stage increased,ratio in Gl stage decreased, to compare with blank group,there is statistical significance(P＜0.001).After simulated by AIgG,to compare with model group,in WBKL serum groups,TGP serum group and prednisone serum group,the MC's ratio in S stage decreased,ratio in G1 stage increased there is statistical significance(P＜0. 05-0.01).Conclusion:the cultured cells from the glomeruli of rat swere glomerular mesangial cells.All the serums have no cytotoxicity.AIgG can stimulate the cells' proliferation,increase the ratio in MC S stage,decrease the ratio in G1 stage.WBKL serum,TGP serum and prednisone serum all can inhibit the proliferation of GMC stimulated by AIgG,and decrease the ratio in stage S,increase the ratio in stage G1.So WBKL can protect the GMC according to impacting the ratio of cell cycle.