Study On The Expression Of Survivin And C-myc Protein In Oral Submucous Fibrosis And Carcinogenesis Of Oral Submucous Fibrosis

Objective: To evaluate expression of Survivin and C-myc protein in Oral Submucous Fibrosis (OSF)and carcinogenesis of Oral Submucous Fibrosis,and to investigate their possible role in carcinogenesis of Oral Submucous Fibrosis.Method: Expression of Survivin and C-myc protein in 45 cases of OSF,15 cases of carcinogenesis of OSF and 10 cases of normal control oral mucosa tissue were studied by SP immunohistochemistry with the Survivin and C-myc rat anti-human monoclonal antibody.The staining intensity for Survivin and C-myc protein were assessmented by average gray-level.Results : (1) Positive Survivin-immunoreactivities were seen in early, middle, late stage OSF and carcinogenesis of OSF, but negative expression in normal control group .The difference between every stage OSF, carcinogenesis of OSF and normal control group was statistically significant(P<0.05).The expression of Survivn at early and middle stages were lower,but still were higher than normal control group,and the expression at late stage increased compared with early and middle stages, the expression at carcinogenesis of OSF was highest. The difference between every stage OSF and carcinogenesis of OSF was statistically significant(P<0.05).(2) Positive C-myc-immunoreactivities were seen in early , middle, late stage OSF and carcinogenesis of OSF,but negative expression in normal control group.The difference between every stage OSF, carcinogenesis of OSF and normal control group was statistically significant(P<0.05).The expression of C-myc at early and middle stages were lower,but still were higher than normal control group,and the expression at late stage increased compared with early and middle stages,the expression at carcinogenesis of OSF was highest. The difference between every stage OSF and carcinogenesis of OSF was statistically significant(P<0.05).(3) The expression of C-myc at early and middle stage OSF was corresponding higher than the expression of Survivin at every stage,the difference was statistically significant(P<0.05).There was no statistically significant difference(P>0.05) in the expression of C-myc and Survivin at late stage OSF and carcinogenesis of OSF. There was positive correlation between the expression of C-myc and Survivin protein in OSF and carcinogenesis of OSF(r=0.693,P<0.05).Their consistency was moderate (P<0.01,k=0.655).There was no statistically significant difference(P>0.05) in diagnosis function.Conclusion: (1) Survivin protein have positive expression in OSF and carcinogenesis of OSF. The frequency of Survivin protein positive expression was obviously higher in carcinogenesis of OSF than in OSF. It may act as an important role in the carcinogenesis of OSF.(2) C-myc protein have positive expression in OSF and carcinogenesis of OSF. The frequency of C-myc protein positive expression was obviously higher in carcinogenesis of OSF than in OSF. It may participate in the carcinogenesis of OSF by binding to promoter of hTERT, subsequently activating the telomerase .(3) Survivin and C-myc protein were correlated positively in OSF and carcinogenesis of OSF, demonstrated that C-myc was probably controlled by Survivin, they may act together promoting the carcinogenesis of OSF.