The Effect Of Extract From Silkworm Chrysalis On The Fatty Acids Spectra Of High-fat Rat's Livers

Object:To study the effect of extract from silkworm chrysalis on the lipids content and fatty acids constituent ratios of high-fat rats.Methods:100 SD rats were randomLy divided into 9 groups according to the serum lipids levels and weihts: normal control group, high-fat control group, positive control group, low, medium and high dose preventive groups, low, medium and high dose therapeutic groups. Different groups were given diffent interventions. The relevant indexes about serum lipids level were tested respectively in the first and the fifth week. The high-fat rats modles were successfully established. In the eleventh week, the rats were killed and their livers were stored in the fridge (-80 ℃).Five livers were taken out from every group randomLy. Lipids were extracted from the livers with Soxhlet. The lipids were esterified and the main fatty acids were determined by gas chromatography according to the follow condition:Inject: 250 ℃,Detect: 260 ℃, FID.30 m × 0.25μm × 0.32 mm (i.d.) FFAP capillary column, the program rate for the fatty acids analysis began at 100℃ for 2 min, increased from 100 ℃ to 200 ℃ at 20 ℃/min, then from 200 ℃ to 210 ℃ at 1 ℃/min, and then from 210 ℃ to 240 ℃ at 3 ℃/min and held for 3 min. Carrier gas wasnitrogen and flow rate was 50 mL/min, spilit ratio was 1 : 40. make up 40mL/min.Results:Compared the lipids contents among all groups, preventative groups therapeutic groups high-fat control group positive control group were different from normal control group. The lipids content in positive control group was more than that in other groups and there was no diffence between preventative groups and therapeutic groups. Comparing the constitute ratios of palmitic acid oleic acid and DHA among the different groups, there was no statistical significance. There was statistical significance between high-fat control group positive control group preventative groups therapeutic groups and normal control group according to myristic acid, but there was statistical significance between preventative groups and therapeutic groups. There was statistical significance between preventative high-dose group normal control group positive control group and therapeutic medium-dose group according to stearic acid constitute ratio. There was statistical significance between preventative medium-dose group and normal control group according to constitute ratio of linoleic acid. Comparing EPA constitute ratio in preventative groups therapeutic groups high-fat control group and normal control group, there was statistical significance; there was statistical significance comparing preventative medium-dose group preventative high-dose group therapeutic groups with positive control group.Conclusions:1. The extract from silkworm chrysalis had reduced lipids contents in the livers of high-fat rats.2. The extract from silkworm chrysalis elevated a few fatty acids constitute ratio in the livers of high-fat rats, such as myristic acid, stearic acid, linoleic acid and EPA.There were no statistical significances among all groups according to the constitute ratios of palmitic acid oleic acid and DHA.