Study On Mechanism Of Vibration-Induced Neural Impairments

Objective After the concept of vibration — induced neuropathy was posed, and research has a breakthrough in both theory and practice. which has been research focus.The mechanism of vibration — induced neuropathy have been done some animal experiments, but up to now which remain to be investigated. It's necessary to make a deep study of vibration on peripheral nervous and central nervous system. So we did the experiment study, for the purpose of investigating the mechanism of vibration-induced neral impairment. This also gives some clues to the further study of diagnostic indicator of vibration — induced neuropathy. So we can achieve the purposes of early diagnosis or treatment.Methods The cleaning grade 24 New Zealand rabbits, with body mass ranging from 2.20kg to 2.50 kg, of either sex, provided by Lukang Party. The rabbits were randomly divided into 4 groups of 6 rabbits(4 males and 4 females): A group (low level of vibration power group), B group (middle level of vibration power), C group (high levels of vibration power group ) and one control group (D group). After the test, were measured in each group.SPSS11.5 software was used to process data.Results (l)With the increase of vibration power, the concentration of serum noradrenalin (NE) , 5-serotonin(5-HT) had a tendency to significantly increase, But the concentration of dopamine (DA) increased firstly , then decreased. Compared with the control group, there was a significant decrease in the concentration of NE in the brain of vibration groups, and their difference was significant (P<0.01) .There was a significant decrease in the concentration of DA in the brain of vibration groups, the concentration of DA in the middle and high vibration power group was significantly different (P<0.01) .(2) Bax and Fas expression were significantly higher than that in the normal controls (P < 0.05) . Bcl-2 in the vibration group was lower than that in the controls, but it had no significance (P > 0.05) (3) Compared with the control group, there was a significant decrease in the concentration of NO and activity NOS in the vibration groups, and their difference was significant (P<0.05) . With increasing vibration power, the concentration of NO and NOS in the brain tissue had a tendency of obvious decrease. Compared with the control group, the concentration of ET in the low vibration power group was not significantly different. However, the concentrations of ET in the high and middle vibration power groups were significantly different compared with the control group. With increasing vibration power, the concentration of ET in the brain tissue had a tendency of obvious increase. Hippocampus of rabbit brain in A group and B group were observed. HSP70 expression was significantly higher than that in the normal controls (P< 0.01) . HSP70 moved from the cytoplasm to the nuleus after vibtation exposure.Conclusions The local vibration may cause monoamine neurotransmitters of central nervous system and peripheral nervous system increase. Central nervous system and peripheral nervous system were out of balance. Local vibration may cause changes of protein related apoptosis and schwanncel cell apoptosis, which indicates that peripheral nervous system was injured. The local vibration may cause the changesof vascular endothelial substance, ATPase activity and HSP70 expression in brain tissue.HSP70 was sensitive biomarkers of vibration; The increase of HSP70 might be regarded as a sign of self-defensive response.