Effect Of Induced HSP70 On The Senescence Of Human Fetal Lung Diploid Fibroblast MRC-5 Cell In The Heat Shock Condition

Objective: To explore the expressive mechanism of induced HSP70 and it' s effect on the senescence of MRC-5 cell in the heat shock condition. Method: MRC-5 cells were treated with heat shock to induce the expression of H SP70 and HSP70 antisense oligonucleotides were transfected to block the expression of HSP70. They were treated at 42℃ for 30min,1h ,2h, 4h respectively and cultured for 3d and 5d with thermal treatment for 1h everyday. The cells were all recovered at 37℃ for 4h and then studied. The expression of HSP70 and apoptosis of cells were detected and assessed by flow cytometry and Superoxide dismutase(SOD) activity was measured. The cells were assessed t hrough senescent associated β -gal and observed under microscopy.Results: Following the heating time increasing, the expression of HSP70 was significantly enhanced in test group than in control group. The activity of SOD was significantly higher in test group than in control group. The SA- β -gal positive rate of MRC-5 cells obviously increased when they were cultured for 3d and 5d in control group.Conclusion: The thermal treatment can successfully induced the expression of HSP70 in MRC-5 cell and induced HSP70 may influence the senescent process of MRC-5 cells by intensifying the ability to remove the oxygen-derived free radicals.