The Empirical Syudy On Anti-Fatigued Of Decoction Of Raising Splen-Yang To Tonify Stomach

Objective:To evaluate the drug action and study the mechanism of action of anti—fatigue of Decotion of raising splen-yang to tonify stomach.Method: Select ninety male mice (through swimming ability preliminary screening), whose body weight are 18-22g.According to the body weight , divide randomly the mice to tree groups,which are blank cotrol group,decotion of raising splen-yang to tonify stomach group, positive control group (spirulina). According to the Department of health study on the test methods for the health food assessment on anti —fatigue effects to experimentize.Tree groups differently are taken distilled water, decoction of raising splenoyang to tonify stomach, spirulina for fifteen days.The lavage amount of 20g weight mouse is 0.4ml.Take experiment after 30min of the end admininstration. Observe the effect of decotion of raising splen-yang to tonify stomach on the time of burden swimming of mice, the increase of LA after 10min swimming of mice, the decreases of LA after 15min rest of mice swimming, blood urea nitrogen, lactic acid dehydrogenase, hepatic glycogen, muscle glycogen to evaluate the drug action and study the mechanism of action of anti —fatigue of Decotion of raising splen-yang to tonify stomach.Results: the results showed that decotion of raising splen-yang to tonify stomach could significantly enhance the exercise tolerance of mice, the time of swimming of decotion of raising splen-yang to tonify stomach group were longer than that of blank control group, the increase of LA after10min swimming of mice of this recipe were lower than that of blank control group, the bloodurea nitrogen level were also lower than that of blank control group, the lactic acid dehydrogenase level of this recipe were higher than that of blank control group.But this recipe has no effect on hepatic giycogen and muscle glycogen level.Conclusion: we can conclude that the decoction of raising splen-yang to tonify stomach has the effectiveness of anti — fatigue and using it for fifteen days can play the anti —fatigue action. This recipe could have the action of anti—fatigue through regulating substance metabolism, enhancing the exercise tolerance and the activity of lactic acid dehydrogenase, accelerating the lactic acid of the muscle to change into pyruvic acid, decreasing the blood lactic acid generation, strengthening the adaptability of mice to loading, decreasing the protein and amino acid resolving under excessive exercise load.