| Hepatitis B virus (HBV) infection is a global health problem, andmore than 2 billion people were infected with HBV, the chronic HBVinfectors are more than 35 million. China has a very high incidence ofhepatitis abut 60 million people by infected. 50% hepatocirrhosis and70-90% liver cancer come on because of the chronic HBV. There are about7-30% HBV carriers infected aberrance of HBV.HBV genotype has any referenced worth on HBV epidemiology,genetics and diagnoses, prognosis.An HBV genotype has been classified as sequences which have asimilarity over the whole genome of abut ≥ 92% or S genome of abut ≥96%. HBV is classified into genotypes A-H, which is important for clinicaland etiological investigation.To establish a simple and practicable method to identify genotypes ofhepatitis B virus, ten primers sets wer designed for six genotypes accordingto genotyped-specific sequence, and used separately for PCR. The genotypeof HBV was identified according to the positive result of PCR. There wasno difference in the genotyping result of by the PCR-RFLP mothed.To understand the distribution of hepatitis B virus genotype in Jilinprovince and its clinical investigation, Based on nest-PCR usingtype-specific primers for the determination of genotype from 194 share ofpositive blood serum in Jilin province.The major genotype in Jilin province was genotype C, and thegenotype BC was a definite proportion.The genotype B was 16 samples , Cwas 172 samples , BC was 6 samples and did not fund others genotypes, theprevalence of genotype B was 8.2%, genotype C was 88.7% and that ofgenotype BC was 3.1%. No other genotypes (such as genotype A, E, F, G,H ) were found.We using restriction fragment length polymorphism (RFLP)-PCR tocheck up the truth of results, we found the results are true and credibility.The prevalence of genotype C showed an increasing trend in chronichepatitis, liver cirrhosis and hepatocellular carcinoma group;in contrast ,the prevalence B showed an opposite trend , although no statisticallysignificant different was observed . The HBeAg positive rate was higher,and the anti-HBe positive rate was lower in patients with chronic genotypeC infection than in genotype B.The prevalence of genotype C contributes to the former group'sprogressive liver disease and showed poor clinical outcomes. |
