The Development And Application Of The Assays For The Detection Of Pathogenic Dermatophytes In Laboratory Animals With Molecular Biological Method

Objective: Laboratory animals, such as mouse, rat, guinea pig, rabbit etc, have been widely used in research, education and industry. Dermatophytes, which cause infection to laboratory animals, are mainly Microsporum canis, Microsporum gypseum and Trichophyton mentagrophytes, which should be absolutely excluded from laborary animals. It is abnormal no matter they are isolated in infectious sites or uninfectious sites, since the dermatophytes in uninfectious sites are also probable causing dermaophytosis of laboratory animals and infect feeders and laboratory members.Currently the laboratory diagnosis of dermatophytes mainly depends on morphological observations, such as direct microscopic observation and dermatophytes culture, which need not only long time but also plenty of experience, incapable of identifying the dermatophytes genera. Then it can not meet the needs of the rapid and accurate detection. Recent years, molecular biological methods, such as RFLP, molecular hybridization, DNA sequencing etc., have been applied in dermatophytes identifications. Particularly, the establishment of...