Study Of Pathogenicity Of 7 Common Nail Pathogenic Fungi And Interaction Of Trichophyton Rubrum And Scopulariopsis Brevicaulis

Objective: To comfirm and compare the pathogenesis of 7 kinds of common or suspicious fungi which may cause onychomycosis.Establish the model to study the interaction of T.rubrum and S.brevicaulis. Methods: 1. Inoculate sterilizd human nails into 3 different mediums of 7 different fungi.Nail plates in each group were observed periodicly by being dissolved with 20% NaOH to compare the pathogensis of different groups. 2. Establishing a nail passing model to compare the passing time of T.rubrum group, S.brevicaulis group and T.rubrum plus S.brevicaulis group. Results: 1.While in Sabouraud agar dishes,some non-dermatophyte molds can invade the nail plates directly as well as T.rubrum. There was no significant difference between T.rubrum and other filamentous fungi such as S.brevicaulis, F. moniliforme, P.funiculosum by comparing the hyphae burden with the method of 20% NaOH dissolving.There was statisticly difference between mycelial fungi and yeast.Calbicans which can form pseudomycelium invade the nails more intensively than R.glutinis. A fungus inoculated in solid SDA is more pathogenic than in Sabouraud broth tube or normal saline tube. 2. S.brevicaulis in the nail passing model took less time to get through the nail plate than T.rubrum.There was no statistic difference between the group of S.brevicaulis and S.brevicaulis puls T.rubrum group.Conclusion:1. Under some conditions, some non-dermatophyte molds can attack the nail plate directly as some dermatophytes. The nail penetration ability of a fungus is proportional to its hyphal formation ability and contact environmental stability.2.In the in vitro nail passing model, The nail passing time of S.brevicaulis had't beening influenced by T.rubrum.