Establishment Of Identification-database Of Common Dermatophytes Using Biolog Automatic Analyzer For Microbes

ObjectiveTo establish identification-database of common Dermatophytes using Biolog Automatic Analyzer for Microbes and investigate application prospect of this system.MethodsDermatophytes were identified to a species level using Phenotype and DNA sequencing. Trichophyton rubrum, Trichophyton mentagrophyte, Trichophyton tonsurans, Microsporum canis, Microsporum gypseum and Epidermophyton floccosum were inoculated into FF microplates and the utilization of 95 different carbon sources were recorded. Growth and reaction spectrum of the strains were described and identification database was set up.ResultsThere are obvious differences of carbon utilization among different strains. Utilization of Raffinose can differentiate Trichophyton mentagrophyte and Trichophyton tonsurans from the other four dermatophytes. Sebacic acid can differentiate Trichophyton mentagrophyte from Trichophyton tonsurans. Meanwhile, Trichophyton rubrum, Microsporum gypseum, Epidermophyton floccosum and Microsporum canis can be differentiated by Fumarate and Succinate. Microsporum gypseum can be identified by using Alanine and Phenylalanine. Utilization of Dextrin can distinguish Epidermophyton floccosum from Microsporum canis.ConclusionsBiolog Automatic Analyzer for Microbes has the ability to identify these six common dermatophytes to species level, so we can speculate the application prospect of this system in identifying different Dermatophyte species.