Rat Model Research Of Yin And Yang Deficiency In Type2 Diabetes Mellitus

Rat Model Research of Yin and Yang Deficiency in Type2 Diabetes MellitusObjective: To build the disease and syndrom combined animal model of Yin and Yang deficiency in type-2 diabetes mellitus, in order to explore the method and standard of Yin and Yang deficiency animal model in type-2 diabetes mellitus.Methods: 60 mice were randomly divided into four groups. Besides the normal control group, other three groups were all given high fat diet. Four weeks later, two of the three latter groups were injected Stroptozotocin to build the diabetes mellitus model—diabetes mellitus blank group and Yin and Yang deficiency group. The latter were built by traditional Chinese herb Huang Bai and Fang Ji for four weeks, then treated by traditional Chinese medicine Jin Kui Shen Qi Wan.Results: The rats of Yin and Yang deficiency group were hyperdrunk, hypersuffered, diuresis, thin, curled up, liked to gather, lazy to move, grew slowly, body hair with no sheen, thin bowl, struggled faintly when given herb, red tongue .In the Yin and Yang deficiency group, the level of serum triglycerides, total cholesterol, low density lipoprotein, plasma cortisol and thyroxin B2 were obviously higher, and the level of insulin, FT3and 6-keto-prostaglandin1α were evidently lower than that in normal control group. All the index signs were instaurated after the modification of Jin Kui Shen Qi Wan.Conclusion: It is available to develop type-2 diabetes mellitus according to the Chinese medicine theory to use the medicine quality of Four Qi and Wu Wei on the base of high fat and Streptozotocin. Through this experiment, animal model of Yin and Yang deficiency and diabetes mellitus are combined, so that the animal model of diabetes mellitus are combined with syndrome, it will provide good conditions to extensively study the diabetes mellitus.