Study On The Safe Margins Of The Proximal And Distal Intestinal Of The Colorectal Cancer

Objective:From histopathology and molecular pathology aspects, to investigate the range of the CRC (colorectal cancer) infiltration in the proximal and distal intestinal. to explore the molecular margin of the proximal and distal intestinal in the CRC and to provide the evidence of the safe margin of the proximal and distal intestinal for the radical operation of CRC.Method:42 CRC patients who underwent radical operation were included in the study. Tumor, normal intestinal and the intestinal within 3cm. from the proximal and distal margins of the tumor were collected from each fresh resected specimen, and the intestinal within 3cm. from the proximal and distal margins of the tumor was sliced transversely every 1cm. each piece was separated into two parts and marked well. In one part, 5μm thick, HE stained serial sections were made for the histopathologic examination. The other part was snap-frozen in -80℃refrigerator for RNA preparation. They were employed to detect by RT-PCR using CEACAM5(formerly CEA) and COX-2 as genetic markers after RNA extraction.Result:1. Found microscopically, the tumor infiltration in the proximal and distal intestinal were 16 of 42 patients (38.1%) and 19 of 42 patients (45.2%) respectively. The infiltration distance was respectively within 2cm in 37 case (88.1%), in 28 case (88.1 %), and within 3cm in all 42 cases.2. The tumor infiltration rates of the proximal and distal intestinal decrease as the distance increases from the tumor. The proximal and distal infiltration and it's distance was correlated with tumor histological type, Dukes'stage and infiltrated depth of intestinal.3. The relative content CEAmRNA and COX-2 mRNA expressions in the tumor,...