Study On The Protection Of Dendrobium Candidum Wall. Exlindl Drop In Acute Radintion-injuried Mice

Objective To study the protection of Dendrobium candidum Wall.exlindl drop (DCWD) on irradiated mice.Methods 125 BALB/c mice were randomly divided into normal control group, irradiation-injured group and DCWD groups which were divided into three groups according to the dose of DCWD. Before whole-body irradiation with 8.0Gy or 5.0Gy 6 MV X-ray, the BALB/c mice were supplied with DCWD once a day for 10 days. After being irradiated, all mice were continued to be fed DCWD until they were died or putted to death. The general state of health, survival time of mice irradiated by 8.0Gy X-ray were observed. The peripheral white blood cells and bone marrow karyocyte of mice irradiated by 5.0Gy X-ray were counted and the index number of spleen were measured, the T-cell subgroup of peripheral blood were determined by flow cytometer. The activity of superoxide dismutase were determined by xanthinoxidase assay and the content of malondialdehyde by thiomalonylurea assay and the pathological change of spleen,liver and lung were detected in mice irradiated by 5.0Gy X-ray.Results (1) General state of health: The vim was poor, food and drink and activity decreased, color pattern was dim and shed easily in mice irradiated by X-ray, especially in mice irradiated by 8.0 Gy X-ray, and aggravated gradually. These symptom was slight and recovery was quicker in irradiated mice by 5.0Gy X-ray. (2) The mean survival time: The mean survival time of mice in each group irradiated by 8.0Gy X-ray were 10 days or less than 10 days, significantly shorter than the normal control group(P<0.01), but the DCWD groups survived longer than irradiation-injured group for 3~4 days(P<0.01);(3) Pathological change of spleen, liver and lung: acute radiation injury of spleen, liver and lung emerged, which appeared blood disturbance of circulation, cell degeneration, comp adaptation reaction. The pathological changes degree was slighter and recovered sooner in...