The Protective Effect Of Ginkgo Biloba Extract And Ginkgolides B On Endothelial Cells

Objective To examination whether endothelium cells (ECs) vulnerable to diabetic serum (DS) and further investigated to whether the protection effects of ginkgolide B (GB) and Ginkgo biloba extracts (GBE) on ECV-304 cell damage induced by DS.Methods1. Made ECs damage model induced by DS. Cultured ECV-304 cells were exposured to10% healthy serum (HS), serum of patient only with diabetes mellitus (DS1), and serum of patient with brain vascular complications of diabetes mellitus (DS2) 30 minutes, 3 hours and 3 days. Proliferative activity of cells was assessed by MTT method. Lactate dehydrogenase (LDH), maleic dialdehyde (MDA) and nitric oxide (NO) concentration were measured in the supernatant of culture medium.2. Atorvastatin (AT)(1, 5, 10μmol/L), GB (1, 5, 10μg/ml), GBE(50, 100, 150μg/ml)and Demethyl sulfoxide (DMSO) were respectively added to ECV-304 cells treat with the culture medium including 10% DS (All results of DS1 groups had no significant difference compared with those of DS2 groups, so DS1 acted as DS in the following experiments). After 3 days, proliferative activity of cells was assessed by MTT method.3. The protective effects of GB and GBE on ECV-304 cell damage by DS. AT acted as positive control and DMSO acted as negative control. Cultured ECV-304 cells were exposure to 10% DS and respectively treated with DMSO (0.1%), AT(5μmol/L), GB(GB10μg/ml), GBE(GBE100μg/ml) for 30 minutes, 3 hours, and 3 days. Cells viability were measured by Typan Blue Staining. LDH, MDA and NO concentration were detected in...