The Myocardial Protection Of Shenfu Injection On Diabetes Mellitus Rats

Objective: To investigate the effect of Shenfu injection on myocardium of living diabetes mellitus rats and the main mechanism. Methods: To establish type 1 diabetes mellitus animal models induced by streptozotocin (60 mg/kg ip),whose fasting blood glucose(FBG) was more than 16.7 mmol/L indicated animal model successful . The control group(C), DM group(C-DM), and SFI treated group(S-DM) had 10 rats respectively. The rats of group S-DM were treated with SFI intraperitoneal injection (10 ml/kg) once a day for 20 weeks. In the end of 20th week, to assay the FBG, TG, TC, FINS, SOD and MDA in serum and myocardium of rats from the three group; to detect the protein expression of GLUT-4 mRNA on myocardial cytomembrane by immunohistochemical assay; to observe the pathologic changes of islets of pancreas and myocardial ultrastructure of the three groups under light and electron microscopes respectively. Results: The percentage of model established of diabetic rats was 90 percent. The level of FBG, TG and TC of rats of group S-DM was lower than that of group C-DM while the level of FINS was highter compared with that of group C-DM (all P<0.01). SOD in serum and myocardium of rats of group S-DM was more than that of group C-DM while MDA was less than that of same group (all P<0.01). The protein expression of GLUT-4 mRNA on cardiac cytomembrane of group S-DM rats was less than that of group C while more than that of group C-DM. Under light microscope, there were much more islet cells in pancreatic slices of group S-DM and they were almost acidophil. However, there were a few islet cells of group C-DM. Quite a few lymphocytes infiltrated in islets of group C-DM and the cytophasm of islet cells was rarefied while some nuclei condensed. The edema on myocardial mitochondria and endothelial cells of cardiac capillary of group C-DM was much more serious than that of group S-DM under electron microscope. Conclusions: SFI protected the healthy islets of pancreas, myocardium and the endothelial cells of cardiac capillary of diabetic rats. There were three mechanisms. First of all,SFI is one of antioxidants. Secondly, SFI protected the healthy islets of pancreas resulting in increase in serum insulin and improvement of metabolism of glucose and lipid of diabetic rats. Thirdly, SFI increased the protein expression of GLUT-4 mRNA on myocardial cytomembane of diabetic rats, which facilitated the transportation of glucose into myocardial cytoplasm beneficial to the metabolism leading to protecting myocardial mitochondria and the endothelia of cardiac capillary and defering the development of cardiomyopathy of diabetes mellitus rats.