Effect Of Fucoidan On The Human Renal Interstitial Fibroblast

Objective : To reveal the mechanism of action that fucoidan counteracts renal interstitium fibrosis,we investigated the effects of fucoidan(FPS) on proliferation , apoptosis and expression of fibronectin(FN),laminin(LN) and transforming growth factorβ1(TGF-β1) in the human renal interstitial fibroblast(HRIF) in vitro.Methods:1. Primary culture and appreciation of HRIF: HRIF was cultured in vitro and detected vimentin,α-actin, cytokeratin, desmin by means of immunofluorescence.2. Drug safety was detected by cytotoxicity test: HRIF incubated with FPS in following concentrations: 25μg/ml, 50μg/ml, 100μg/ml, 200μg/ml. After 24 hours and 48 hours, cells were stained by trypan blue and computed rate of living cells/total cells.3. Cell proliferation was detected by MTT assay.4. The levels of FN,LN,TGF-β1 in supernatant were detected by ELISA respectively.5. Cell apoptosis was detected by flow cytometry.Results:1. Character of the cells cultured in vitro: fusiform, vimentin andα-actin were positive(+),cytokeratin and desmin were negative (-).2. HRIF was incubated with FPS for 24 hours or 48 hours, living cells'rate of each group was above 94%.