| Objective To observe the effects of Laminin and Fibronectin on dermal component-free hair follicles and culture hair outer root sheath cells of human in vitro, in order to understand the control of hair follicle growth.Methods Organ culture model of dermal component-free hair follicles was established in the study. The effects of Laminin and Fibronectin on dermal free hair follicle were observed by micrometer,confocal microscopy and electron microscope. It was also established in the study that outer root sheath cells of hair cultured in vitro. It was studied that adhesion and proliferation of the cells influenced by Laminin and Fibronectin. The expression of Laminin and Fibronectin receptors in outer root sheath cell clones was detected by immunocytochemical staining.Results①The complete dermal component-free hair follicles were isolated and their culture model in vitro was established successfully;②The length of dermal component-free hair follicles did not change in all groups after being cultured 30 days , and there were no differences among them(P>0.05).Hair follicle epithelial cells grew from the middle(inferior) part and hair bulb of dermal component-free hair follicles in groups which coated with Laminin and Fibronectin;③Confocal microscopy and electron microscope studies showed that survival epithelial of dermal component-free hair follicles in test groups was much more than control groups after 6,12,18 days culture(P<0.05). Fn group was the better one(P<0.05).④ORSC was isolated successfully and multiplied 8～9 passages;⑤The adhesion and proliferation of ORSC were improved in Ln and Fn groups(P<0.05),Fn group was the better one(P<0.05). ⑥The expression of Laminin and Fibronectin receptors in ORSC clones was positive.CONCLUSION1. It shows that Ln and Fn cannot efficiently accelerate the growth of human derma component-free hair follicle in vitro,but they can prolong its survival time and it is better in Fn group.2. Ln and Fn can promote ORSC adhesion and proliferation and it is better in Fn group. |
