Study On Extracting The Active Components Of Thesium Chinense Turcz. And Its Effect Mechanism

Thesium Chinense Turcz. is a Santalaceae plant, belongs to the herbage or the frutex, is also a rare arbor plant, takes autoeciousness or semiparasite as its main biology characteristic. The whole grass may be used as medicine, has the antibiotic action and diminish inflammation effects, be provided with clearing away heat and detoxifcation function, et al. Thesium Chinense Turcz. has the high medical value as one kind of a broad-spectrum antibiotic Chinese herbal medicine. As a result of being restricted by the thoughts and the methods of the traditional Chinese herbal medicine research, the function of Thesium Chinense Turcz. and its mechanism is not confirmed, which limited its'application in the clinical. In view of the fact of the developing characteristic of modern Chinese herbal medicine, that the functional mechanism of Chinese native herbal medicine is the hot spot in the current pharmacology research. Therefore, effectively extracting the active components, and making clear its effect mechanism is the vital significance to study Thesium Chinense Turcz.The active components of Thesium Chinense Turcz were extracted by Ultrasonic-wave extraction (UE) and Mro-wave-assisted (MAE) technology in the research, Through bacterial susceptibility test, two extraction reagent, water and alcohol were compareted, which mostly be used to extract the components of Chinese herbal medicine. The results indicated that using double wave 216nm and 276nm of ultraviolet ray to examine the extracting can judge availably the effect of the active components,and water-extract in Mro-wave-assisted is the better method to extract the active components in Thesium Chinense Turcz..In order to verified the susceptibility of Thesium Chinense Turcz to different bacterium, Seven kinds of common nosogenesis-bacteria were used to do bacterial examination test by the the method of Oxford-cup tests in the experiment. The antibacterial experiment conditions and the parameter were also studied. The results showed that:Thesium Chinense Turcz. is sensitive to both the G+ and the G-- bacterium, and the effect on the G+ was stronger than that on the G--, the sensitive order of seven kinds of experimental bacterium was S.aureus, A.hydrophila, B.cereus, S.lutea, B.subtilis, and P.aeruginosa. And, the S.aureus and A.hydrophila were the most sensitive bacterium, but E.coli is the worst to Thesium Chinense Turcz.. Each sensitive bacteriums'MIC was also tested in the experiment. The result confirmed that Thesium Chinense Turcz. is available to use as a broad-spectrum antibiotic Chinese herbal medicine in clinic. The antibacterial mechanism of Thesium Chinense Turcz was studied using its active components which were extracted in the former experiment. The sugar and the protein metabolism on the S.aureus were tested by biochemistry examination methods. The result indicated that the active components of Thesium Chinense Turcz. could change the bacterium metabolism mechanism. The effect disturbs or destroys the oxidation reduction condition of the S.aureus, thus affect its metabolism. In addition, chromatographic analysis technology was used to separate active components of Thesium Chinense Turcz, and five kinds of components were obtained to do antibacterial experiment to acquire their correlation. In conclusion, the antibacterial function of Thesium Chinense Turcz. is not the single medicine's action, but the joint action of its several kinds of ingredients. The test laid an important foundation to continue advanced research on the Chinese herbal medicine Thesium Chinense Turcz.