| Human genome is composed of 22 autosomes and 2 sexchromosomes, which is the DNA 1 meter in length containing 30hundred million nucleotides, encode 30 ~ 40 thousand genes andtakes the whole inherited informat-ion correlating with growth,development,decrepitude and death of human anybody. Differentindividuals share the same number of genes and the same sequenceof nucleotide. Theresistant structure of genome ensures theconcomitant and stability of human as a species, but the majorpremise of biocenotic evolution is inherited variances, Any newvariance originates from a mutation, namely a change in sequence ofDNA base. Mutations can cause allele with abnormal functioncontinuously, advantaged variances are kept and passed down byelecting. At the same time, human evolution led to the differencesand polymorphisms of genome among different races, groups andindividuals.Human genome project (HGP) was started up in due form in1990,.Checking variances of genic structure was an importantcontent of HGP. Polymorphisms of DNA forming in the process ofbiocenotic evolution defines at least two different phenotypes orgenotypes existin DNA molecule among groups, it means that therearesome differences or variances in sequence of DNAnucleotide atthe same location on the same chromosomeamong individuals.Single-nucleotide polymorphisms (SNP)is a sort of DNApolymorphisms, existing in genome widely,according to estimationthere will be more than one SNP in per one thousand nucleotides, sothere are over 3 million SNPs in whole human genome, and about200 thousand SNPs locate in coding regions. Lander E inMassachusetts Institute of Technology (MIT) put forward and namedSNP as the third inherited marker in1996. Because SNPs take a lot ofinformation correlating with susceptibility of disease,the extensiveapplication of SNP made it as a new means to study diversity ofgenome and identify or locate genesconnecting with diseases.Pregnancy-induced hypertension (PIH) is a characteristiccomplication during gestation, the pathogenyis unclear. It's basicpathologic change is all of small arteries spasm and damage ofendotheliocytes, subsequently led tohypertension, proteinuria andedema . Some study shows that the vWF circulating level ofhypertensive disorder complicating pregnancy was higher than thatin normal pregnancy. von Willebrand factor gene MspIpolymorphism in intron 19 might play a role by increasing vWFcirculating level .In order to ascertain the relationship between vonWillebrand factor gene MspI polymorphism in intron 19 andhypertensive disorder complicating pregnancy among Chinses.The cases selection : 70patients with hypertensive disordercomplicating pregnancy, all were inpatients of ChangchunObstetrical and Gynecological Hospital and the second jilinuniversity form October 2003 to October 2005 were collected anddivided into 3 groups in according to the criteria of diagnose andclassification for hypertensive disorder complicating pregnancy inthe sixth edition "Obstetrics and Gynecology". 27 cases cases weremild preeclampsia patients, the gestational period was 38.6±1.4weeks, the age was 27.4±2.2 years old, There was no significantdifference in gestational period and age. (P>0 05);32 cases weresevere preeclampsia patients , the gestational period was 35.2±3.5weeks, the age was 27.9±3.7 years old. There was significantdifference in gestational period (P<0 .001). 11 cases were eclampsiapatients, the gestational period was 35.7±3.0 weeks, the age was28.7±4.8 years old. There was significant difference in gestationalperiod (P<0 .001).82 normal gravida, the gestational period was39.2±0.9weeks, the age was 27.9±2.6 years old, withoutcomplication of pregnancy were recruited as control. All patientshave no primary liver and kidney disease, no history of diabetesmellitus,rheumatism and tumors and have not used any drugs .Methods: Collected fasting venous blood 2 ml in the morning,then reserved them in -20℃. MspI polymorphism in intron 19 wasevaluated both in constitutive and tissue DNA by PCR amplification,specific endonuclease digestion, and then detection with agarose orpolyacrylamide gel electrophoresis.Statistics analysis: Using t test,F test,regression analysisand correlation analysis.Results: Comparing with normal gravida group, the Msp Igenotypedistributions in hypertensive disorder complicating pregnancywere different from that of the healthy controls(x2=24.0008(P<0.05). The observed allele frequencies deviate from the expectedHardy-Weinberg distribution in our patient population.Thefrequencies of the M-/M-genotypes in hypertensive disordercomplicating pregnancy groups were lower than that in thenormal.von willebrand factor gene MspI polymor-phism in intron 19was closely related with hypertensive disorder complicatingpregnancy . ( P 〈 0.05 〉 . Among the healthy controls ,thefrequencies of the M+ and M-genotypes were 0.19 and0.0.815,respectively.there were 0.457 and 0.543 in hypertensivedisorder complicating pregnancy groups, respectively. There wassignificant difference in the frequencies of genotype(P<0 .05).Thefrequencies of the M+ genotypes in severe preeclampsia patients andeclampsia patients were significant higher than that in the normaland mild preeclampsia patients,. (P〈0.05〉. The results indicatethat M+ allele frequencies were closely related with the stages ofhypertensive disorder complicating pregnancy.Conclusion : MspI polymorphism in intron 19 in the vonWillebrand factor gene correlate with the occurrence and thestages of hypertensive disorder complicating pregnancy amongChinses.
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