| Objective: To study the expression of Bone MorPhogenetic Proteins (BMP-2) and Transforming Growth Factor-β2(TGF-β2), exploring the regularity and the function of BMP-2 and TGF-β2 in the process of comeal thermal burns in rats.Methods: 42 Wistar white rats (weightl70210g )were divided into seven groups randomly .The models of severe comeal burns were induced with thermal metal in rats.The comeal were surgically removed at different time point (6h,24h,3d,5d,1 w,2w) after operation .Six rats for each time .The localization and expression of BMP-2 and TGF-β2 were determined by using immunochemical methods .Mean gray degree of the positive cell of staining section was measured by computer image processing software.Results: The expression of BMP-2 and TGF-β2 were viewed in normal corneals of rats , and the BMP-2 average gray degree of positive cell of 24h ,3,5d group in burn comeal is lower than that of normal group (P<0.05 ) . The TGF-β2 average gray degree of positive cell of 6h ,24h,3,5d group in burn comeal is also lower than that of normal group(P<0.05),and their expression change correlate with each other( r=0.584,P<0.01).Conclusion: BMP-2 and TGF-β2 may be a pair of synergia factors according to the result of experiment, both involving in the process of corneal thermal burns cell proliferation and apoptosis. BMP-2 and TGF-β2 maybe involve in the corneal wound healing,this indicate that BMP-2 and TGF-β2 may do co-administration in the corneal wound healing.
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