Study Osteopontin On The Pathogenesis Of Endometriosis

OBJECTIVETo study the expression of osteopontin(OPN) in the eutopic endometrium and in the ectopic endometrium of women with endometriosis. To investigate the role of osteopontin on the pathogenesis of endometriosis.METHODForty patients with endometriosis were selected as study group (EM), fifteen patients without endometriosis were selected as control group (EN). Patients with endometriosis were grouped by Revised American Fertility Society (rAFS) stages, 6 patients at Ⅰ and Ⅱ stages, 22 patients at Ⅲ stages and 12 patients at Ⅳ stages. In our study group, 2 red peritoneal endometriotic lesion(RPL) and 2 uterosacral ligament nodules(ULN) were taken from 2 patients with peritoneal endometriosis;38 specimens of ovarian endometrioma, 16 matched eutopic endometrium (Eu), 9 Eu at proliferative stage, 7 Eu at secretory stage were taken from 38 patients with ovarian endometriosis. In the control group, 15 specimens of endometrium, 8 at proliferative stage and 7 at secretory stage were biopsied from 15 patients without endometriosis. The expression of OPN messenger RNA (mRNA) in the ectopic and eutopic endometrium were detected by QT-PCR.The ratio of OPN Ct value/β -Actin C_t value is theaccurate concentration of OPN mRNA, the ratio is lower, the expression of OPN mRNA is higher.The means of cycle threshold is the cycle when the fluorescence single in each reactive tube reached the pre-defined threshold. RESULTS( 1) The expression of OPN messenger RNA in the eutopic and ectopic endometrium from the study group and the endometrium from the control group was detected by QT-PCR. The expression of OPN mRNA in the eutopic endometrium(l. 12 + 0.07) from the study group was higher than that of in the endometrium (1.25 ±0.12)from the control group, P<0.01. The expression of OPN mRNA in the ectopic endometrium(1.02 ±0.11) was higher than that of in the eutopic endometrium(1.12 ±0.07) in the study group, P<0.01.( 2 ) The expression of OPN mRNA was detected from the endometrium in the study group and control group during proliferative stage and secretory stage. There was no obvious different during the proliferative stage(1.13±0.08) and the secretory stage(1.12±0.06) in the study group, P>0.05, The expression of OPN mRNA was no relationship with menstrul cycle in the study group;The expression of OPN mRNA in the endometrium was higher during the secretory stage(l .13±0.04) than that of during the proliferative stage(l .36±0.04), it was related to menstrul cycle in the control group.;The expression of OPN mRNA in the study group(1.13±0.08) was higher than that of in the control group(1.36±0.04) during the proliferative stage, P<0.01;There was no obvious different in the expression of OPN mRNA between the study group(1.12±0.06)and the control group(1.13±0.04) during the secretory stage, P>0.05.(3) The expression of OPN mRNA in I and II stages(1.18±0.03) III stages(1.06±0.08) IVstages(0.87±0.08) was obviously different, P<0.01, it was increased with the rAFS stages. The expression level of OPNmRNA was correlated with rAFS stages. CONCLUSIONSThe OPN mRNA is over expressed in the eutopic and ectopic endometrium from patients with endometriosis, it was correlated with rAFS stages and increased with the stages, but no relationship with menstrul cycle, which may play some roles on the pathogenesis of endometriosis.