The Concentration Of Hepatocyte Growth Factor And Soluble Intercellular Adhesion Molecule-1 In Cerebrospinal Fluid In Central Nervous System Disease

Objective To determine cerebrospinal fluid(CSF) concentration of hepatocyte growth factor(HGF)and soluble intercellular adhesion molecule-l(sICAM-1) in different central nervous system diseases, and to explore the relationship between the CSF concentration of HGF and sICAM-1 with different central nervous system diseases.Methods The concentrations of HGF and sICAM-1 in CSF were measured by the enzyme-linked immunosorbent assay(ELISA) in different central nervous system diseases bacterial meningitis (14), viral encephalitis (17), cerebral infarction (12), cerebral hemorrhage (16) and neurosis, migraineand , tension headache (10). The last 10 patients were chosen as control.What's more, the concentrations of HGF and sICAM-1 in CSF in intracranial infection were analysed separately for correlation with cells or protein in CSF.Results 1. In intracranial infection, the concentrations of HGF and sICAM-1 in CSF of bacterial meningitis patients were 0.95±0.61ng/ml, 14.67 ± 7.42 ng/ml, which were significantly higher than those in control group (P<0.01 ) .The concentrations of HGF and sICAM-1 in CSF of viral encephalitis patients were 0.59 ±0.51 ng/ml 7.83 ± 5.75 ng/ml, which were higher than those in control group(P<0.05) .In addtion, the difference between the two meningitis groups were significant (P<0.05) .2. In intracranial infection, the concentrations of HGF and sICAM-1 in CSF had no relationship with CSF cells or protein (P>0.05 ) .3. In cerebral infarction group, the concentrations of HGF and sICAM-1 in CSF were 0.64 ± 0.41 ng/ml, 9.41 ± 7.10 ng/ml, which were significantly higher than control group (P<0.01 P<0.05) .In cerebral hemorrhage group,the concentrations of HGF and sICAM-1 in CSF were 0.62 + 0.32 ng/ml, 7.97 + 5.08 ng/ml ,which were significantly higher than control group (P<0.01 P<0.05) too. The concentrations of HGF and sICAM-1 between the two acute cerebrovascular diseases were no difference.Conclusions 1.In intracranial infection, the concentrations of HGF and sICAM-1 in CSF were higher in the diseases of bacterial meningitis and viral encephalitis, but had no relationship with the levels of cells or protein in CSF.Moreover, the concentrations of HGF and sICAM-1 in bacterial meningitis were higher than those in viral encephalitis. So the concentrations of HGF and sICAM-1 in CSF might serve as markers of diagnosis and differential diagnosis of bacterial meningitis and viral encephalitis.2. In acute cerebrovascular disease, the levels of HGF and sICAM-1 in CSF were upregulated. The inflammation factor may introduce the overpresson of HGF. HGF might play a role of protection to blood vessel endodermis cell as neurotrophic factor. The overpression of sICAM-1 may participate generation of acute cerebrovascular disease as inflammational/immunizational factor.The index may provide foundation for gene therapy.